Effect of Petiveria alliacea Extracts on Metabolism of K562 Myeloid Leukemia Cells
Abstract
:1. Introduction
2. Results
2.1. The Esperanza Extract Reduces the Cell Proliferation Rate without Inducing Apoptosis
2.2. Alterations in the Endometabolome of the K562 Cell Line Treated with Esperanza Extract
3. Discussion
4. Materials and Methods
4.1. Plant Material and Extraction
4.2. In Vitro Cytotoxicity and Proliferation Assays
4.3. Cell Death Evaluation
4.4. OCR and ECAR Evaluation
4.5. ATP Determination
4.6. Untargeted Metabolomics Analysis
4.6.1. Sample Preparation
4.6.2. Untargeted Metabolomics by LC-QTOF-MS
4.6.3. Untargeted Metabolomics by GC-QTOF-MS
4.6.4. Quality Control Samples
4.7. Data Processing and Analysis
4.8. Annotation of Statistically Significant Molecular Features
4.9. Altered Metabolite Pathway Mapping
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Rojas, L.; Pardo-Rodriguez, D.; Urueña, C.; Lasso, P.; Arévalo, C.; Cala, M.P.; Fiorentino, S. Effect of Petiveria alliacea Extracts on Metabolism of K562 Myeloid Leukemia Cells. Int. J. Mol. Sci. 2023, 24, 17418. https://doi.org/10.3390/ijms242417418
Rojas L, Pardo-Rodriguez D, Urueña C, Lasso P, Arévalo C, Cala MP, Fiorentino S. Effect of Petiveria alliacea Extracts on Metabolism of K562 Myeloid Leukemia Cells. International Journal of Molecular Sciences. 2023; 24(24):17418. https://doi.org/10.3390/ijms242417418
Chicago/Turabian StyleRojas, Laura, Daniel Pardo-Rodriguez, Claudia Urueña, Paola Lasso, Cindy Arévalo, Mónica P. Cala, and Susana Fiorentino. 2023. "Effect of Petiveria alliacea Extracts on Metabolism of K562 Myeloid Leukemia Cells" International Journal of Molecular Sciences 24, no. 24: 17418. https://doi.org/10.3390/ijms242417418