The Impact of Various Organic Phosphorus Carriers on the Uptake and Use Efficiency in Barley
Abstract
:1. Introduction
2. Results
2.1. The Growth of Barley under Diverse OP Treatments
2.2. The Growth of Barley Nourished with Different Concentrations of Phytin
2.3. Utilization of P in Barley Nourished with Different Concentrations of Phytin
2.4. Acid Phosphatase Activity and Phytase Activity of Barley
2.5. Phylogenetic Analysis of PAPs and PHTs in Barley
2.6. Expression of P-Utilization-Related Genes
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Experimental Design
4.3. Analysis of Plant Growth
4.4. Determination of P Content and Enzyme Activity
4.5. Phylogenetic Analysis
4.6. Real-Time PCR
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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OP | Length (cm) | Surface Area (cm2) | Average Diam. (mm) | Volume (mm3) | ||||
---|---|---|---|---|---|---|---|---|
CN4027 | Baudin | CN4027 | Baudin | CN4027 | Baudin | CN4027 | Baudin | |
Pi | 29.5 ± 0.6c | 19.3 ± 1.3d | 4.23 ± 0.50bc | 4.24 ± 0.68e | 0.38 ± 0.01a | 0.38 ± 0.01bc | 40.3 ± 3.9b | 24.5 ± 4.5c |
Phytin | 30.8 ± 0.1c | 49.5 ± 2.9b | 3.72 ± 0.32c | 7.00 ± 0.73bc | 0.36 ± 0.03a | 0.44 ± 0.02b | 36.0 ± 6.0b | 58.5 ± 8.5b |
ATP | 42.2 ± 3.6b | 60.2 ± 3.7ab | 5.43 ± 0.33ab | 9.37 ± 0.33a | 0.40 ± 0.03a | 0.35 ± 0.02c | 23.5 ± 0.5c | 57.8 ± 1.0b |
RNA | 31.9 ± 1.5c | 27.1 ± 5.1cd | 3.34 ± 0.39cd | 4.85 ± 0.46de | 0.37 ± 0.04a | 0.72 ± 0.02a | 34.0 ± 3.2bc | 84.3 ± 6.8a |
F6P | 33.6 ± 3.7c | 63.8 ± 3.2a | 4.02 ± 0.57c | 8.79 ± 0.48a | 0.38 ± 0.02a | 0.44 ± 0.03b | 43.5 ± 2.5b | 89.5 ± 8.5a |
IHP | 18.1 ± 0.3d | 53.3 ± 4.1ab | 2.35 ± 0.16d | 7.99 ± 0.80ab | 0.39 ± 0.04a | 0.45 ± 0.03b | 24.7 ± 3.4c | 95.7 ± 8.0a |
Treatment | Length | Surface Area | Volume | |||
---|---|---|---|---|---|---|
CN4027 | Baudin | CN4027 | Baudin | CN4027 | Baudin | |
Pi | 4.68 ± 0.36b | 2.97 ± 0.40c | 1.06 ± 0.09a | 0.63 ± 0.16c | 17.7 ± 1.4a | 11.8 ± 2.6e |
Phytin | 3.92 ± 0.23c | 10.3 ± 0.1b | 0.68 ± 0.14c | 3.35 ± 0.52ab | 12.4 ± 1.7b | 42.0 ± 5.6bc |
ATP | 3.29 ± 0.05d | 10.6 ± 0.5b | 0.76 ± 0.01bc | 2.92 ± 0.24ab | 17.1 ± 0.5a | 31.9 ± 2.8d |
RNA | 3.95 ± 0.30c | 11.0 ± 1.1b | 0.51 ± 0.06d | 3.01 ± 0.58ab | 13.8 ± 0.4ab | 47.7 ± 3.0ab |
F6P | 2.83 ± 0.22d | 13.9 ± 0.9a | 0.74 ± 0.09bc | 3.70 ± 0.35a | 13.6 ± 1.2ab | 58.0 ± 4.6a |
IHP | 3.41 ± 0.03cd | 14.5 ± 0.2a | 0.75 ± 0.06bc | 3.78 ± 0.27a | 12.3 ± 2.6b | 57.9 ± 5.7a |
Treatment | Length | Surface Area | Volume | |||
---|---|---|---|---|---|---|
CN4027 | Baudin | CN4027 | Baudin | CN4027 | Baudin | |
Pi | 24.8 ± 0.2b | 16.3 ± 0.9cd | 3.17 ± 0.56c | 3.55 ± 0.52c | 22.6 ± 2.6c | 12.7 ± 1.9d |
Phytin | 26.8 ± 0.1b | 39.3 ± 2.8b | 3.04 ± 0.18c | 3.65 ± 0.21c | 23.6 ± 4.3bc | 16.5 ± 2.9d |
ATP | 38.9 ± 3.6a | 49.6 ± 3.2a | 4.67 ± 0.34a | 6.45 ± 0.09a | 26.4 ± 4.6d | 26.0 ± 1.7c |
RNA | 27.9 ± 1.3b | 16.1 ± 4.0d | 2.88 ± 0.33c | 1.84 ± 0.33d | 20.2 ± 2.9c | 36.7 ± 3.9ab |
F6P | 30.8 ± 3.6b | 49.9 ± 2.6a | 3.28 ± 0.47bc | 5.10 ± 0.12b | 29.9 ± 1.3b | 31.5 ± 3.9abc |
IHP | 14.7 ± 0.3c | 38.8 ± 3.9b | 1.60 ± 0.11d | 4.20 ± 0.53bc | 12.2 ± 1.5d | 37.7 ± 2.6a |
Phytin Treatments | Length (cm) | Surface Area (cm2) | Average Diam (mm) | Volume (mm3) | ||||
---|---|---|---|---|---|---|---|---|
CN4027 | Baudin | CN4027 | Baudin | CN4027 | Baudin | Baudin | CN4027 | |
−P | 56.6 ± 1.6c | 78.2 ± 3.8b | 9.37 ± 0.24b | 12.38 ± 0.56b | 0.49 ± 0.04a | 0.51 ± 0.03a | 127 ± 7a | 148 ± 13ab |
LP | 48.4 ± 2.4d | 51.9 ± 2.1c | 6.08 ± 0.50d | 7.31 ± 0.53d | 0.39 ± 0.01b | 0.36 ± 0.01c | 58.7 ± 6.7d | 61.5 ± 1.8d |
NP | 36.9 ± 2.5e | 50.9 ± 0.3c | 4.86 ± 0.19e | 8.74 ± 0.05cd | 0.38 ± 0.01b | 0.41 ± 0.01bc | 46.7 ± 2.7e | 67.3 ± 1.3d |
H1P | 64.7 ± 3.2b | 72.8 ± 2.0b | 8.23 ± 0.35bc | 10.34 ± 0.30c | 0.41 ± 0.00b | 0.47 ± 0.04ab | 83.7 ± 2.8c | 142 ± 12b |
H2P | 58.2 ± 3.0bc | 78.7 ± 2.2b | 7.55 ± 0.53c | 9.76 ± 0.52c | 0.41 ± 0.01b | 0.41 ± 0.03bc | 72.5 ± 4.0c | 93.7 ± 5.6c |
H3P | 80.7 ± 1.2a | 122 ± 5a | 10.6 ± 0.5a | 17.5 ± 1.0a | 0.42 ± 0.01b | 0.46 ± 0.01ab | 104 ± 2b | 171 ± 10a |
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Huo, Y.; Wang, J.; Xu, Y.; Hu, D.; Zhang, K.; Chen, B.; Wu, Y.; Liu, J.; Yan, T.; Li, Y.; et al. The Impact of Various Organic Phosphorus Carriers on the Uptake and Use Efficiency in Barley. Int. J. Mol. Sci. 2023, 24, 17191. https://doi.org/10.3390/ijms242417191
Huo Y, Wang J, Xu Y, Hu D, Zhang K, Chen B, Wu Y, Liu J, Yan T, Li Y, et al. The Impact of Various Organic Phosphorus Carriers on the Uptake and Use Efficiency in Barley. International Journal of Molecular Sciences. 2023; 24(24):17191. https://doi.org/10.3390/ijms242417191
Chicago/Turabian StyleHuo, Yuanfeng, Jingyue Wang, Yinggang Xu, Deyi Hu, Kexian Zhang, Bingjie Chen, Yueyi Wu, Jiaxin Liu, Tianlang Yan, Yang Li, and et al. 2023. "The Impact of Various Organic Phosphorus Carriers on the Uptake and Use Efficiency in Barley" International Journal of Molecular Sciences 24, no. 24: 17191. https://doi.org/10.3390/ijms242417191