Integrative Analysis of Metabolome and Transcriptome Provides Insights into the Mechanism of Flower Induction in Pineapple (Ananas comosus (L.) Merr.) by Ethephon
Abstract
:1. Introduction
2. Results
2.1. Overview of Metabolite Profiles during Flower Induction
2.2. Transcriptome Analyses
2.3. Functional Annotation of Differentially Expressed Genes
2.4. Integrative Analysis of DAMs and DEGs
2.5. Verification of DEGs by qPCR
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Treatments
4.2. Metabolomic Analysis
4.2.1. Sample Preparation and LC–ESI-MS/MS System Analysis
4.2.2. Differentially Accumulated Metabolites Analysis
4.3. RNA-seq Analysis
4.3.1. RNA Isolation and Library Construction
4.3.2. Differentially Expressed Genes (DEGs) Analysis
4.4. Association Analysis between Transcriptome and Metabolome
4.5. qRT-PCR Verification of Gene Expression
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
BL | ‘Comte de Paris’ |
qRT-PCR | Real-time quantitative reverse transcription polymerase chain reaction |
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Lin, W.; Liu, S.; Xiao, X.; Sun, W.; Lu, X.; Gao, Y.; He, J.; Zhu, Z.; Wu, Q.; Zhang, X. Integrative Analysis of Metabolome and Transcriptome Provides Insights into the Mechanism of Flower Induction in Pineapple (Ananas comosus (L.) Merr.) by Ethephon. Int. J. Mol. Sci. 2023, 24, 17133. https://doi.org/10.3390/ijms242417133
Lin W, Liu S, Xiao X, Sun W, Lu X, Gao Y, He J, Zhu Z, Wu Q, Zhang X. Integrative Analysis of Metabolome and Transcriptome Provides Insights into the Mechanism of Flower Induction in Pineapple (Ananas comosus (L.) Merr.) by Ethephon. International Journal of Molecular Sciences. 2023; 24(24):17133. https://doi.org/10.3390/ijms242417133
Chicago/Turabian StyleLin, Wenqiu, Shenghui Liu, Xiou Xiao, Weisheng Sun, Xinhua Lu, Yuyao Gao, Junjun He, Zhuying Zhu, Qingsong Wu, and Xiumei Zhang. 2023. "Integrative Analysis of Metabolome and Transcriptome Provides Insights into the Mechanism of Flower Induction in Pineapple (Ananas comosus (L.) Merr.) by Ethephon" International Journal of Molecular Sciences 24, no. 24: 17133. https://doi.org/10.3390/ijms242417133