Skin-Microbiome Assembly in Preterm Infants during the First Three Weeks of Life and Impact of Topical Coconut Oil Application
Abstract
:1. Introduction
2. Results
2.1. Succession of the Skin Microbiota of Preterm Infants in Both Control and Intervention Infants
2.2. Factors Associated with Diversity of the Preterm-Infant Skin Microbiome
2.3. Effect of Topical Coconut Oil on the Skin Microbiome
3. Discussion
4. Materials and Methods
4.1. Participant Recruitment and Sample Collection
4.2. DNA Extraction, PCR, and Amplicon Sequencing
4.3. Bioinformatics and Statistical Analysis
4.4. Quantification and Detection of Staphylococcus spp.
4.5. Quantification and Detection of Bifidobacterium spp.
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Characteristics | Control Arm (n = 28) | Intervention Arm (n = 28) |
---|---|---|
Gestation (weeks) | 28 (23.7–29.5) | 27.6 (24.1–29.3) |
Birth weight (g) | 984 (424–1534) | 1040 (590–1420) |
Caesarean delivery | 18 | 15 |
Male | 13 | 19 |
Taxon | Reference | Condition | Coefficient | q-Value |
---|---|---|---|---|
Corynebacterium | Axilla | Groin | −2.31 | 0.0001 |
Clostridium | Axilla | Groin | −1.84 | 9.46 × 10−5 |
Streptococcus | Axilla | Groin | 1.77 | 0.002 |
Staphylococcus | Day 1 | Day 7 | 1.68 | 2.43 × 10−9 |
Staphylococcus | Day 1 | Day 14 | 1.71 | 2.18 × 10−9 |
Bifidobacterium | Day 1 | Day 7 | 1.88 | 0.006 |
Cutibacterium | Day 1 | Day 14 | −1.88 | 0.0003 |
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Ghori, N.-U.-H.; Mullally, C.A.; Nicol, M.P.; Currie, A.; Hibbert, J.; Payne, M.S.; Patole, S.; Strunk, T. Skin-Microbiome Assembly in Preterm Infants during the First Three Weeks of Life and Impact of Topical Coconut Oil Application. Int. J. Mol. Sci. 2023, 24, 16626. https://doi.org/10.3390/ijms242316626
Ghori N-U-H, Mullally CA, Nicol MP, Currie A, Hibbert J, Payne MS, Patole S, Strunk T. Skin-Microbiome Assembly in Preterm Infants during the First Three Weeks of Life and Impact of Topical Coconut Oil Application. International Journal of Molecular Sciences. 2023; 24(23):16626. https://doi.org/10.3390/ijms242316626
Chicago/Turabian StyleGhori, Noor-Ul-Huda, Christopher A. Mullally, Mark P. Nicol, Andrew Currie, Julie Hibbert, Matthew S. Payne, Sanjay Patole, and Tobias Strunk. 2023. "Skin-Microbiome Assembly in Preterm Infants during the First Three Weeks of Life and Impact of Topical Coconut Oil Application" International Journal of Molecular Sciences 24, no. 23: 16626. https://doi.org/10.3390/ijms242316626