The Controversial Effect of Antibiotics on Methicillin-Sensitive S. aureus: A Comparative In Vitro Study
Abstract
:1. Introduction
2. Results
2.1. S. aureus Strains 6850 and JB1 Were Susceptibile to All Antibiotic Combinations during Planktonic Growth
2.2. Antibiotic Combinations Containing Rifampicin Effectively Killed Intracellular Bacteria but Not the Intracellular SCV S. aureus JB1
2.3. Antibiotic Combinations with Rifampicin Promote the Selection of SCVs
2.4. Antibiotics Successfully Halt Biofilm Formation in S. aureus WT and SCV
2.5. Successful Biofilm Eradication via Rifampicin and Fosfomycin Antibiotic Combinations, JB1 Biofilms Respond Solely to Rifampicin
2.6. The Zeta Potential of All Strains Was Less Negative When Preincubated with Antibiotics
3. Discussion
4. Material and Methods
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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(A) | ||||
Antibiotics | 6850 MIC | JB1 MIC | ||
Cefazolin | 0.5 | 0.08 ± 0.05 | ||
Flucloxacillin | 0.17 ± 0.09 | 0.09 ± 0.04 | ||
Fosfomycin | 0.85 ± 0.69 | 0.4 ± 0.2 | ||
Rifampicin | 0.015 | 0.015 | ||
(B) | ||||
Antibiotic Combinations | 6850 MIC | 6850 FICI | JB1 MIC | JB1 FICI |
Cefazolin + fosfomycin | 0.5 ± 0.01/ 1 ± 0.26 | indifferent | 0.16 ± 0.1/ 0.12 ± 0.11 | indifferent |
Cefazolin + rifampicin | 0.08 ± 0.08/ 0.03 ± 0.02 | indifferent | 0.1 ± 0.5/ 0.002 ± 0.001 | synergistic |
Flucloxacillin + fosfomycin | 0.375 ± 0.323/ 1 ± 1.3 | indifferent | 0.17 ± 0.1/ 0.35 ± 0.36 | indifferent |
Flucloxacillin + rifampicin | 0.05 ± 0.02/ 0.015 ± 0.008 | synergistic | 0.15 ± 0.09/ 0.008 ± 0.006 | indifferent |
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Hackemann, V.C.J.; Hagel, S.; Jandt, K.D.; Rödel, J.; Löffler, B.; Tuchscherr, L. The Controversial Effect of Antibiotics on Methicillin-Sensitive S. aureus: A Comparative In Vitro Study. Int. J. Mol. Sci. 2023, 24, 16308. https://doi.org/10.3390/ijms242216308
Hackemann VCJ, Hagel S, Jandt KD, Rödel J, Löffler B, Tuchscherr L. The Controversial Effect of Antibiotics on Methicillin-Sensitive S. aureus: A Comparative In Vitro Study. International Journal of Molecular Sciences. 2023; 24(22):16308. https://doi.org/10.3390/ijms242216308
Chicago/Turabian StyleHackemann, Valeria C. J., Stefan Hagel, Klaus D. Jandt, Jürgen Rödel, Bettina Löffler, and Lorena Tuchscherr. 2023. "The Controversial Effect of Antibiotics on Methicillin-Sensitive S. aureus: A Comparative In Vitro Study" International Journal of Molecular Sciences 24, no. 22: 16308. https://doi.org/10.3390/ijms242216308