Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells
Abstract
:1. Introduction
2. Results
2.1. Changes in Cell Motility and Proliferation
2.2. Changes in mRNA Levels
2.3. Changes in lncRNA Levels
3. Discussion
3.1. The PI3K/AKT Pathway
3.2. The MAPK/ERK Pathway
3.3. Epithelial Mesenchymal Transition
3.4. Podosome Formation
3.5. Proliferation, Senescence and Apoptosis
4. Materials and Methods
4.1. Cell Culture
4.2. Cell Proliferation Assay
4.3. Cell Migration Assay
4.4. Transwell Invasion Assay
4.5. Viability Assay
4.6. Total RNA Extraction and Real-Time Quantitative PCR
4.7. RNA Sequencing
4.8. RNA Sequencing Data Analysis
4.9. Protein Extraction and Western Blot Analysis
4.10. Immunocytochemistry and Fluorescence Microscopy
4.11. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Jacksi, M.; Schad, E.; Buday, L.; Tantos, A. Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells. Int. J. Mol. Sci. 2023, 24, 1310. https://doi.org/10.3390/ijms24021310
Jacksi M, Schad E, Buday L, Tantos A. Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells. International Journal of Molecular Sciences. 2023; 24(2):1310. https://doi.org/10.3390/ijms24021310
Chicago/Turabian StyleJacksi, Mevan, Eva Schad, László Buday, and Agnes Tantos. 2023. "Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells" International Journal of Molecular Sciences 24, no. 2: 1310. https://doi.org/10.3390/ijms24021310
APA StyleJacksi, M., Schad, E., Buday, L., & Tantos, A. (2023). Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells. International Journal of Molecular Sciences, 24(2), 1310. https://doi.org/10.3390/ijms24021310