Role of the Neuroendocrine System of Marine Bivalves in Their Response to Hypoxia
Round 1
Reviewer 1 Report
This manuscript proposes an extensive review on role of the neuroendocrine system of marine bivalves in response to hypoxia, the neuroendocrine system and its related signaling systems play crucial roles in the regulation of various physiological and behavioral processes in mollusks and can affect hypoxia tolerance. The neuro-endocrine stress response to hypoxia and the neuro-endocrine regulation of oxygen homeostasis have been summarized based on the current knowledge. The findings in the cited materials show that the marine mollusks’ tolerance to hypoxia is provided by the integration of various signaling systems and their activation causes changes in the expression of neurotransmitters such as DA, 5-HT, CHAT, and NO. As such, the matter is of interest, but this article still has some shortcomings as follows.
1. The references should not be appeared in the abstract.
2. Table 1 cannot be found in the manuscript.
3. In line 177-179, no references are cited.
4. In Figure 3, Ca (DA, E, NE) should be CAs (DA, E, and NE)
5. In line 229-231, there are only references for vertebrates
(Bacqué-Cazenave et al., 2020), and no references for invertebrates are cited.
6. The statement in the sentence is inconsistent with the figure in regarding the level of 5-HT, as can be seen from the figure, the level of 5-HT is reduced, but in the sentence, But the description is increased in the sentence in line 244
7.Many species that are not marine bivalves are mentioned，for example in line 613, 616, there are a lot of references that have been done on Marine bivalves, therefore, it is recommended to find references related to marine bivalves.
8. “Copmlex” should be complex in Figure 5
Author Response
Comments and Suggestions for Authors
This manuscript proposes an extensive review on role of the neuroendocrine system of marine bivalves in response to hypoxia, the neuroendocrine system and its related signaling systems play crucial roles in the regulation of various physiological and behavioral processes in mollusks and can affect hypoxia tolerance. The neuro-endocrine stress response to hypoxia and the neuro-endocrine regulation of oxygen homeostasis have been summarized based on the current knowledge. The findings in the cited materials show that the marine mollusks’ tolerance to hypoxia is provided by the integration of various signaling systems and their activation causes changes in the expression of neurotransmitters such as DA, 5-HT, CHAT, and NO. As such, the matter is of interest, but this article still has some shortcomings as follows.
Reply
Dear reviewer. We express our great gratitude to you for review of the MS and careful work on MS. We have reviewed all your valuable comments and made appropriate corrections in the text and figures. We hope that we have satisfied all your comments.
Best wishes
Vyacheslav Dyachuk
Elena Kotsyuba
1. The references should not be appeared in the abstract.
Reply
Sure, I have deleted all eferences in the abstract of the MS
- Table 1 cannot be found in the manuscript.
Reply
Sorry, this is our mistake. The text of the MS does not contain any Tables
- In line 177-179, no references are cited.
Reply
Malham et al., 2002; Chen et al., 2008 are cited in MS
- In Figure 3, Ca (DA, E, NE) should be CAs (DA, E, and NE)
Reply
We have changed
- In line 229-231, there are only references for vertebrates
(Bacqué-Cazenave et al., 2020), and no references for invertebrates are cited.
Reply
Agree! we have added some Refs for invertebrates
- The statement in the sentence is inconsistent with the figure in regarding the level of 5-HT, as can be seen from the figure, the level of 5-HT is reduced, but in the sentence, But the description is increased in the sentence in line 244
Reply
Right! Hypoxia exposure causes the 5-HT-immunoreactivity level to decrease in the ganglia and increase in gills and other not nerve tissues. We have changed.
7.Many species that are not marine bivalves are mentioned，for example in line 613, 616, there are a lot of references that have been done on Marine bivalves, therefore, it is recommended to find references related to marine bivalves.
Reply
We agree. We have added two paragraphs (line 613-622)
- “Copmlex” should be complex in Figure 5
Reply
Done
Thanks for the revision
Best wishes
Dr Vyacheslav Dyachuk
Dr Elena Kotsyuba
Author Response File: 
Author Response.docx
Reviewer 2 Report
A thorough review, well done. The manuscript needs only minor copy editing to improve clarity and readability. A copy of the PDF with my editorial suggests is attached.
Comments for author File: 
Comments.pdf
Author Response
Dear Reviewer!
We want to express huge thanks to the reviewer who did a great job to improve this manuscript. Thank you for your time and a detailed correction of MS.
WE have taken into account all your comments and corrected the text.
Best
Vyacheslav Dyachuk
Ekena Kotsyuba
Author Response File: Author Response.doc
Round 2
Reviewer 1 Report
no more comments.
