Characterization and Localization of Sol g 2.1 Protein from Solenopsis geminata Fire Ant Venom in the Central Nervous System of Injected Crickets (Acheta domestica)
Abstract
:1. Introduction
2. Results
2.1. Expression, Purification, and Identification of rSol g 2.1 Protein
2.2. Secondary Structure of Sol g 2.1
2.3. Paralysis Symptoms and Aggressive Behavior
2.4. Specific Localization of Sol g 2.1 Protein with Immunofluorescence Analysis
2.5. Immunoblotting
2.6. The Overall Structure and Structural Homology of Sol g 2.1 Protein
2.7. Sol g 2.1 Protein—Octopamine Receptor Docking
3. Discussion
4. Materials and Methods
4.1. Expression and Purification of Recombinant Sol g 2.1
4.2. Identification of rSol g 2.1 Protein Using LC-MS/MS
4.3. Characterization of rSol g 2.1 Protein
4.4. Paralysis Activity
4.5. Specific Localization of Sol g 2.1 Protein with Immunofluorescence
4.6. Immunoblotting
4.7. Homology Modeling
4.8. Protein—Protein Docking
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Band | Theoretical MW 1 | MW 2 | Experiment MW 3 | Matched Peptide Sequences 4 | Score XC 5 | Homologous Molecule |
---|---|---|---|---|---|---|
Purified band | 15,966 Da | 13,386.68 Da | 17 kDa | R.AGVVETTVLAR.E K.AINITGCILR.A | 155 | Venom allergen 2; S. invicta |
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Nonkhwao, S.; Rungsa, P.; Buraphaka, H.; Klaynongsruang, S.; Daduang, J.; Kornthong, N.; Daduang, S. Characterization and Localization of Sol g 2.1 Protein from Solenopsis geminata Fire Ant Venom in the Central Nervous System of Injected Crickets (Acheta domestica). Int. J. Mol. Sci. 2023, 24, 14814. https://doi.org/10.3390/ijms241914814
Nonkhwao S, Rungsa P, Buraphaka H, Klaynongsruang S, Daduang J, Kornthong N, Daduang S. Characterization and Localization of Sol g 2.1 Protein from Solenopsis geminata Fire Ant Venom in the Central Nervous System of Injected Crickets (Acheta domestica). International Journal of Molecular Sciences. 2023; 24(19):14814. https://doi.org/10.3390/ijms241914814
Chicago/Turabian StyleNonkhwao, Siriporn, Prapenpuksiri Rungsa, Hathairat Buraphaka, Sompong Klaynongsruang, Jureerut Daduang, Napamanee Kornthong, and Sakda Daduang. 2023. "Characterization and Localization of Sol g 2.1 Protein from Solenopsis geminata Fire Ant Venom in the Central Nervous System of Injected Crickets (Acheta domestica)" International Journal of Molecular Sciences 24, no. 19: 14814. https://doi.org/10.3390/ijms241914814