Immunosuppressive Activity of Exosomes from Granulocytic Myeloid-Derived Suppressor Cells in a Murine Model of Immune Bone Marrow Failure
Abstract
:1. Introduction
2. Results
2.1. G-MDSC-exos Confirmation and Quantification
2.2. Suppression of T-Cell Proliferation In Vitro
2.3. Attenuation of Immune BMF In Vivo
2.4. Modulation of miRNA Delivery into CD4+ and CD8+ T Cells
3. Discussion
4. Materials and Methods
4.1. Animals and Exosome Preparation
4.2. T-Cell Proliferation In Vitro
4.3. Induction of AA/BMF and G-MDSC Exosome Therapy
4.4. Cell Counts and Flow Cytometry
4.5. Cytokine Measurement
4.6. Cell Sorting and RNA Isolation
4.7. Quantitative Real-Time RT-PCR (RT-qPCR)
4.8. Statistics
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Manley, A.L.; Chen, J.; Fitzgerald, W.; Feng, X.; Young, N.S. Immunosuppressive Activity of Exosomes from Granulocytic Myeloid-Derived Suppressor Cells in a Murine Model of Immune Bone Marrow Failure. Int. J. Mol. Sci. 2023, 24, 14661. https://doi.org/10.3390/ijms241914661
Manley AL, Chen J, Fitzgerald W, Feng X, Young NS. Immunosuppressive Activity of Exosomes from Granulocytic Myeloid-Derived Suppressor Cells in a Murine Model of Immune Bone Marrow Failure. International Journal of Molecular Sciences. 2023; 24(19):14661. https://doi.org/10.3390/ijms241914661
Chicago/Turabian StyleManley, Ash Lee, Jichun Chen, Wendy Fitzgerald, Xingmin Feng, and Neal S. Young. 2023. "Immunosuppressive Activity of Exosomes from Granulocytic Myeloid-Derived Suppressor Cells in a Murine Model of Immune Bone Marrow Failure" International Journal of Molecular Sciences 24, no. 19: 14661. https://doi.org/10.3390/ijms241914661