Folate Receptor Targeted Photodynamic Therapy: A Novel Way to Stimulate Anti-Tumor Immune Response in Intraperitoneal Ovarian Cancer
Abstract
:1. Introduction
2. Results
2.1. In Vitro Results
2.1.1. Evaluation of OVCAR3-Luc Viability upon PDT
2.2. In Vivo Results
2.2.1. Development of a Humanized In Vivo Mouse Model of Intraperitoneal Ovarian Cancer
2.2.2. Before the Reconstitution of Immune System
- Evaluation of the PS Uptake within Targeting Tissue
- Determination of the Fractionized Illumination Scheme
- Evaluation of PDT Efficacy
- Evaluation of the Cytokine Release upon PDT
2.2.3. After the Reconstitution of Immune System
- Evaluation of PDT Efficacy
- Evaluation of the Immune Cell Response upon PDT
- Evaluation of the Cytokines’ Release upon PDT
3. Discussion
4. Materials and Methods
4.1. In Vitro
4.1.1. Cell Culture
4.1.2. Luciferase Transfection
4.1.3. Luciferase Assay
4.1.4. Photosensitizer
4.1.5. Photodynamic Therapy In Vitro
4.1.6. Viability Assays
4.2. In Vivo
4.2.1. Animals
4.2.2. Development of a Humanized Mouse Mode of Intraperitoneal Ovarian Cancer
4.2.3. Before the Reconstitution of Immune System (Non-Reconstituted (NR) Mice)
- PS Uptake
- Determination of the Fractionized Illumination Scheme
- Photodynamic Therapy Protocol
- ELISA
4.2.4. After the Reconstitution of Immune System (Reconstituted (R) Mice)
- Reconstitution of Immune System
- Photodynamic Therapy Protocol
- Flow Cytometric Analysis
- High Sensitivity Human Cytokine Magnetic Bead Kit
4.3. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Baydoun, M.; Boidin, L.; Leroux, B.; Vignion-Dewalle, A.-S.; Quilbe, A.; Grolez, G.P.; Azaïs, H.; Frochot, C.; Moralès, O.; Delhem, N. Folate Receptor Targeted Photodynamic Therapy: A Novel Way to Stimulate Anti-Tumor Immune Response in Intraperitoneal Ovarian Cancer. Int. J. Mol. Sci. 2023, 24, 11288. https://doi.org/10.3390/ijms241411288
Baydoun M, Boidin L, Leroux B, Vignion-Dewalle A-S, Quilbe A, Grolez GP, Azaïs H, Frochot C, Moralès O, Delhem N. Folate Receptor Targeted Photodynamic Therapy: A Novel Way to Stimulate Anti-Tumor Immune Response in Intraperitoneal Ovarian Cancer. International Journal of Molecular Sciences. 2023; 24(14):11288. https://doi.org/10.3390/ijms241411288
Chicago/Turabian StyleBaydoun, Martha, Léa Boidin, Bertrand Leroux, Anne-Sophie Vignion-Dewalle, Alexandre Quilbe, Guillaume Paul Grolez, Henri Azaïs, Céline Frochot, Olivier Moralès, and Nadira Delhem. 2023. "Folate Receptor Targeted Photodynamic Therapy: A Novel Way to Stimulate Anti-Tumor Immune Response in Intraperitoneal Ovarian Cancer" International Journal of Molecular Sciences 24, no. 14: 11288. https://doi.org/10.3390/ijms241411288