Effects of Follicular Fluid on Physiological Characteristics and Differentiation of Fallopian Tube Epithelial Cells Implicating for Ovarian Cancer Pathogenesis
Abstract
:1. Introduction
2. Results
2.1. The Effect of Porcine Follicular Fluid (PFF) on the Primary FTECs
2.2. The Effect of PFF on the Organoid Formation in 3D Matrigel Culture
2.3. The Effect of PFF on the Differentiation of FTECs in ALI Culture
2.4. Simulating the Direct Stimulation of the Oviduct Epithelium by FF
3. Discussion
4. Materials and Methods
4.1. Isolation and Culture of FTECs
4.2. Collection of Porcine Follicular Fluid (PFF)
4.3. 3D and ALI Culture of FTECs
4.4. Immunofluorescence Staining
4.5. Western Blot Analysis
4.6. Analysis of Proliferation Competency
4.7. TUNEL Assay
4.8. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhu, M.; Wang, N.; Wang, S.; Wang, Y.; Yang, X.; Fan, J.; Chen, Y. Effects of Follicular Fluid on Physiological Characteristics and Differentiation of Fallopian Tube Epithelial Cells Implicating for Ovarian Cancer Pathogenesis. Int. J. Mol. Sci. 2023, 24, 10154. https://doi.org/10.3390/ijms241210154
Zhu M, Wang N, Wang S, Wang Y, Yang X, Fan J, Chen Y. Effects of Follicular Fluid on Physiological Characteristics and Differentiation of Fallopian Tube Epithelial Cells Implicating for Ovarian Cancer Pathogenesis. International Journal of Molecular Sciences. 2023; 24(12):10154. https://doi.org/10.3390/ijms241210154
Chicago/Turabian StyleZhu, Maobi, Na Wang, Sha Wang, Yao Wang, Xiawen Yang, Jianglin Fan, and Yajie Chen. 2023. "Effects of Follicular Fluid on Physiological Characteristics and Differentiation of Fallopian Tube Epithelial Cells Implicating for Ovarian Cancer Pathogenesis" International Journal of Molecular Sciences 24, no. 12: 10154. https://doi.org/10.3390/ijms241210154