Endogenous TOM20 Proximity Labeling: A Swiss-Knife for the Study of Mitochondrial Proteins in Human Cells
Abstract
:1. Introduction
2. Results
2.1. Characterization of Mitochondrial Post- and Co-Translational Import Reporters
2.2. LARP4 Does Not Participate in the Co-Translational Import of preOTC-DHFR Reporter
2.3. TOM20 Proxisome Characterization as a Strategy to Identify Effectors of the Mitochondrial Co-Translational Import
2.4. TOM20-mTb-Based BioID Unravels New Proteins Localized in the Mitochondria
2.5. TOM20-mTb as a Tool to Detect Protein Entry Inside Mitochondria
3. Discussion
4. Materials and Methods
4.1. Isolation of Primary Fibroblasts
4.2. Cell Culture
4.3. Generation of KO Cell Lines
4.4. Generation of Endogenous BioID Cell Lines
4.5. Repair Template Plasmids and Reporters Cloning
4.6. Proximity Labeling
4.7. Mass Spectrometry Analyses
4.8. Cell Transfection with Reporter Constructs
4.9. Immunofluorescence Analysis and Confocal Microscopy Observation
4.10. Image Analysis
4.11. Western Blotting
4.12. Immunoprecipitation
4.13. Data Analyses
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Meurant, S.; Mauclet, L.; Dieu, M.; Arnould, T.; Eyckerman, S.; Renard, P. Endogenous TOM20 Proximity Labeling: A Swiss-Knife for the Study of Mitochondrial Proteins in Human Cells. Int. J. Mol. Sci. 2023, 24, 9604. https://doi.org/10.3390/ijms24119604
Meurant S, Mauclet L, Dieu M, Arnould T, Eyckerman S, Renard P. Endogenous TOM20 Proximity Labeling: A Swiss-Knife for the Study of Mitochondrial Proteins in Human Cells. International Journal of Molecular Sciences. 2023; 24(11):9604. https://doi.org/10.3390/ijms24119604
Chicago/Turabian StyleMeurant, Sébastien, Lorris Mauclet, Marc Dieu, Thierry Arnould, Sven Eyckerman, and Patricia Renard. 2023. "Endogenous TOM20 Proximity Labeling: A Swiss-Knife for the Study of Mitochondrial Proteins in Human Cells" International Journal of Molecular Sciences 24, no. 11: 9604. https://doi.org/10.3390/ijms24119604