Δ8-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study
Abstract
:1. Introduction
2. Results
2.1. Differentiation of SH-SY5Y Cells with RA
2.2. Transcriptomic Analysis
3. Discussion
4. Materials and Methods
4.1. Synthesis and Purification of Δ8-THC
4.2. Cell Culture, Differentiation and Treatment with Δ8-THC
4.3. Western Blot Analysis for Tyrosine Hydroxylase
4.4. RNA Extraction and Library Preparation
4.5. Sequencing Analysis
4.6. Comparative Analysis and In Silico Inspection
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | CTRL | Δ8-THC | log2 Fold Change | q-Value | Protein |
---|---|---|---|---|---|
GLS2 | 120.39 | 151.17 | 0.33 | 2.38 × 10−2 | GLS |
GRIA1 | 494.82 | 561.49 | 0.18 | 1.19 × 10−2 | AMPA receptor |
GRIA2 | 1928.70 | 2098.12 | 0.12 | 8.32 × 10−4 | |
GRIA3 | 1727.64 | 1915.39 | 0.15 | 8.36 × 10−5 | |
GRIA4 | 541.77 | 745.06 | 0.46 | 1.46 × 10−13 | |
GRIN2C | 2.41 | 13.29 | 2.46 | 4.22 × 10−3 | NMDA receptor |
HOMER2 | 816.27 | 1007.53 | 0.30 | 6.95 × 10−9 | Homer |
SHANK3 | 1790.25 | 1910.40 | 0.09 | 1.52 × 10−2 | SHANK |
ITPR1 | 9393.09 | 9664.99 | 0.04 | 1.53 × 10−2 | IP3R |
ITPR2 | 1542.24 | 1645.44 | 0.09 | 2.57 × 10−2 | |
PLCB1 | 1167.82 | 1269.17 | 0.12 | 1.16 × 10−2 | PLC |
PLD2 | 1390.54 | 1577.33 | 0.18 | 1.25 × 10−5 | PLD |
PRKCA | 8513.01 | 9226.42 | 0.12 | 1.58 × 10−12 | PKC |
PRKCG | 87.89 | 136.22 | 0.63 | 6.35 × 10−5 |
Gene | CTRL | Δ8-THC | log2 Fold Change | q-Value | Synapsis |
---|---|---|---|---|---|
GABRA3 | 551.40 | 616.31 | 0.16 | 2.03 × 10−2 | Gabaergic |
GABRG3 | 50.57 | 98.84 | 0.97 | 1.12 × 10−6 | Gabaergic |
GABRR2 | 30.10 | 17.44 | −0.79 | 2.27 × 10−2 | Gabaergic |
CHRNA3 | 3521.51 | 3364.80 | −0.07 | 2.01 × 10−2 | Cholinergic |
CHRNA6 | 15.65 | 1.66 | −3.24 | 1.60 × 10−4 | Cholinergic |
CHRNA7 | 2073.17 | 1844.79 | −0.17 | 2.26 × 10−6 | Cholinergic |
CHRNB2 | 9219.72 | 8367.57 | −0.14 | 5.31 × 10−18 | Cholinergic |
PRKACB | 3247.01 | 3042.53 | −0.09 | 1.17 × 10−3 | Cholinergic |
MAPK1 | 1856.47 | 1716.04 | −0.11 | 3.28 × 10−3 | Cholinergic |
CREB3L3 | 13.24 | 4.98 | −1.41 | 1.67 × 10−2 | Cholinergic |
CREB3L4 | 1438.70 | 1281.63 | −0.17 | 1.24 × 10−4 | Cholinergic |
CREB5 | 2677.55 | 2544.16 | −0.07 | 2.34 × 10−2 | Cholinergic |
SLC18A3 | 280.52 | 213.47 | −0.39 | 1.11 × 10−4 | Cholinergic |
DRD4 | 108.35 | 54.82 | −0.98 | 3.20 × 10−8 | Dopaminergic |
CALY | 7.22 | 1.66 | −2.12 | 2.62 × 10−2 | Dopaminergic |
DDC | 13,066.29 | 12,497.37 | −0.06 | 4.46 × 10−6 | Dopaminergic |
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Anchesi, I.; Schepici, G.; Chiricosta, L.; Gugliandolo, A.; Salamone, S.; Caprioglio, D.; Pollastro, F.; Mazzon, E. Δ8-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study. Int. J. Mol. Sci. 2023, 24, 9486. https://doi.org/10.3390/ijms24119486
Anchesi I, Schepici G, Chiricosta L, Gugliandolo A, Salamone S, Caprioglio D, Pollastro F, Mazzon E. Δ8-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study. International Journal of Molecular Sciences. 2023; 24(11):9486. https://doi.org/10.3390/ijms24119486
Chicago/Turabian StyleAnchesi, Ivan, Giovanni Schepici, Luigi Chiricosta, Agnese Gugliandolo, Stefano Salamone, Diego Caprioglio, Federica Pollastro, and Emanuela Mazzon. 2023. "Δ8-THC Induces Up-Regulation of Glutamatergic Pathway Genes in Differentiated SH-SY5Y: A Transcriptomic Study" International Journal of Molecular Sciences 24, no. 11: 9486. https://doi.org/10.3390/ijms24119486