miR-196b-5p and miR-107 Expression Differentiates Ocular Sebaceous Carcinoma from Squamous Cell Carcinoma of the Conjunctiva
Abstract
:1. Introduction
2. Results
2.1. Patient Characteristics
2.2. MicroRNA Analysis
2.2.1. MicroRNA Expression Differentiates Normal Epithelium from Tumor
2.2.2. No Prognostic microRNAs for Metastatic SCCC or Recurrent OSC
2.2.3. Differentiating OSC from SCCC
3. Discussion
4. Materials and Methods
4.1. Patients and Samples
4.2. RNA Isolation
4.3. MicroRNA Expression Profiling
4.4. Validation MicroRNA Expression
4.5. Predicting OSC Status Using ROC Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Controls | SCCC | OSC | ||
---|---|---|---|---|
Gender | m | 6 | 8 | 7 |
f | 6 | 10 | 7 | |
median age at diagnosis [range] | 75 [59–85] | 50.5 [36–89] | 75 [48–91] | |
median follow up [range in months] | 39 [6–120] | 34 [12–156] | ||
Muir–Torre Syndrome | 0 | 0 | ||
Lynch Syndrome | 1 | 0 | ||
TNM at diagnosis | T1 | 7 | 4 | |
T2 | 4 | 3 | ||
T3 | 1 | 6 | ||
T4 | 6 | 1 | ||
N | 6 | 2 | ||
M | 5 | 0 | ||
Follow up: | ||||
exenteration | 6 | 5 | ||
metastases | 6 | 3 | ||
recurrences | 2 | 8 | ||
death by disease | 5 | 3 | ||
prognosis bad | 7 | 8 | ||
prognosis good | 11 | 6 |
SCCC | OSC | ||
---|---|---|---|
Gender | m | 5 | 7 |
f | 7 | 4 | |
median age at diagnosis [range] | 56.5 [31–88] | 69 [48–88] | |
median follow up [range in months] | 24.6 [3–85] | 36 [2–122] | |
Muir–Torre Syndrome | 0 | 0 | |
Lynch Syndrome | 0 | 0 | |
TNM at diagnosis | T1 | 6 | 0 |
T2 | 4 | 7 | |
T3 | 1 | 3 | |
T4 | 1 | 1 | |
N | 1 | 0 | |
M | 0 | 0 | |
Follow up: | |||
exenteration | 2 | 4 | |
metastases | 0 | 1 | |
recurrences | 5 | 4 | |
death by disease | 0 | 1 |
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de Keizer, R.O.B.; Vriends, A.L.M.; Hötte, G.J.; Paridaens, D.A.; Wiemer, E.A.C.; Verdijk, R.M. miR-196b-5p and miR-107 Expression Differentiates Ocular Sebaceous Carcinoma from Squamous Cell Carcinoma of the Conjunctiva. Int. J. Mol. Sci. 2022, 23, 4877. https://doi.org/10.3390/ijms23094877
de Keizer ROB, Vriends ALM, Hötte GJ, Paridaens DA, Wiemer EAC, Verdijk RM. miR-196b-5p and miR-107 Expression Differentiates Ocular Sebaceous Carcinoma from Squamous Cell Carcinoma of the Conjunctiva. International Journal of Molecular Sciences. 2022; 23(9):4877. https://doi.org/10.3390/ijms23094877
Chicago/Turabian Stylede Keizer, Ronald O. B., Anne L. M. Vriends, Gijsbert J. Hötte, Dion A. Paridaens, Erik A. C. Wiemer, and Robert M. Verdijk. 2022. "miR-196b-5p and miR-107 Expression Differentiates Ocular Sebaceous Carcinoma from Squamous Cell Carcinoma of the Conjunctiva" International Journal of Molecular Sciences 23, no. 9: 4877. https://doi.org/10.3390/ijms23094877