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Article

Characterization of the Skeletal Muscle Proteome in Undernourished Old Rats

1
Human Nutrition Unit, INRAE, Auvergne Human Nutrition Research Center, Clermont Auvergne University, 63000 Clermont-Ferrand, France
2
Animal Products Quality Unit (QuaPA), INRAE, 63122 Clermont-Ferrand, France
3
Metabolomic and Proteomic Exploration Facility, Clermont Auvergne University, INRAE, 63122 Clermont-Ferrand, France
4
Department of Clinical Nutrition, Clermont-Ferrand University Hospital Center, 63000 Clermont-Ferrand, France
*
Author to whom correspondence should be addressed.
Academic Editors: Marta Anna Szychlinska and Rosario Barone
Int. J. Mol. Sci. 2022, 23(9), 4762; https://doi.org/10.3390/ijms23094762
Received: 16 March 2022 / Revised: 10 April 2022 / Accepted: 15 April 2022 / Published: 26 April 2022
(This article belongs to the Special Issue Highlights in Pathophysiology of the Musculoskeletal System)
Aging is associated with a progressive loss of skeletal muscle mass and function termed sarcopenia. Various metabolic alterations that occur with aging also increase the risk of undernutrition, which can worsen age-related sarcopenia. However, the impact of undernutrition on aged skeletal muscle remains largely under-researched. To build a deeper understanding of the cellular and molecular mechanisms underlying age-related sarcopenia, we characterized the undernutrition-induced changes in the skeletal muscle proteome in old rats. For this study, 20-month-old male rats were fed 50% or 100% of their spontaneous intake for 12 weeks, and proteomic analysis was performed on both slow- and fast-twitch muscles. Proteomic profiling of undernourished aged skeletal muscle revealed that undernutrition has profound effects on muscle proteome independently of its effect on muscle mass. Undernutrition-induced changes in muscle proteome appear to be muscle-type-specific: slow-twitch muscle showed a broad pattern of differential expression in proteins important for energy metabolism, whereas fast-twitch muscle mainly showed changes in protein turnover between undernourished and control rats. This first proteomic analysis of undernourished aged skeletal muscle provides new molecular-level insight to explain phenotypic changes in undernourished aged muscle. We anticipate this work as a starting point to define new biomarkers associated with undernutrition-induced muscle loss in the elderly. View Full-Text
Keywords: skeletal muscle; aging; sarcopenia; undernutrition; proteome skeletal muscle; aging; sarcopenia; undernutrition; proteome
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MDPI and ACS Style

Barbé, C.; Salles, J.; Chambon, C.; Giraudet, C.; Sanchez, P.; Patrac, V.; Denis, P.; Boirie, Y.; Walrand, S.; Gueugneau, M. Characterization of the Skeletal Muscle Proteome in Undernourished Old Rats. Int. J. Mol. Sci. 2022, 23, 4762. https://doi.org/10.3390/ijms23094762

AMA Style

Barbé C, Salles J, Chambon C, Giraudet C, Sanchez P, Patrac V, Denis P, Boirie Y, Walrand S, Gueugneau M. Characterization of the Skeletal Muscle Proteome in Undernourished Old Rats. International Journal of Molecular Sciences. 2022; 23(9):4762. https://doi.org/10.3390/ijms23094762

Chicago/Turabian Style

Barbé, Caroline, Jérôme Salles, Christophe Chambon, Christophe Giraudet, Phelipe Sanchez, Véronique Patrac, Philippe Denis, Yves Boirie, Stéphane Walrand, and Marine Gueugneau. 2022. "Characterization of the Skeletal Muscle Proteome in Undernourished Old Rats" International Journal of Molecular Sciences 23, no. 9: 4762. https://doi.org/10.3390/ijms23094762

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