Genome-Wide Identification and Expression Analysis of the 14-3-3 Gene Family in Mango (Mangifera indica L.)
Abstract
:1. Introduction
2. Results
2.1. Genome-Wide Identification of Mi14-3-3 Gene Family Members and Characterization of Their Proteins
2.2. Multiple Sequence Alignment of Mi14-3-3 Proteins
2.3. Gene structure and Motif Analysis
2.4. Chromosome Distribution of 14-3-3 Gene Family Members in Mango
2.5. Phylogenetic Analysis of the Mi14-3-3 Genes
2.6. Analysis of the Cis-Regulatory Elements of Mi14-3-3 Gene Family Member Promoters
2.7. Synteny Analysis of Mi14-3-3 Genes
2.8. Expression Profiles of Mi14-3-3 Genes in Different Tissues
2.9. Expression Patterns of Mi14-3-3s under Abiotic Stress
3. Discussion
4. Materials and Methods
4.1. Materials and Treatments
4.2. Identification and Sequence Analysis of 14-3-3 Gene Family Members in Mango
4.3. Chromosomal Location and Gene Structure Analyses
4.4. Sequence Alignment and Phylogenetic Analyses
4.5. Prediction of Cis-Regulatory Elements
4.6. Tandem Duplication and Synteny Analysis
4.7. RNA Extraction and qRT–PCR
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board statement
Informed consent statement
Data availability statement
Conflicts of Interest
References
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Gene Name | Gene Locus | Chr | aa | pI | II | MW (Da) | Gene Entry Number |
---|---|---|---|---|---|---|---|
Mi14-3-3-A1 | 15934509- 15936351 | Chr11 | 257 | 4.78 | 47.14 | 29,051.64 | OK491862 |
Mi14-3-3-A2 | 8935256- 8937263 | Chr12 | 262 | 4.74 | 48.11 | 29,631.29 | OK491863 |
Mi14-3-3-B1 | 4489787- 4491126 | Chr10 | 252 | 4.83 | 37.22 | 28,431.38 | OK491864 |
Mi14-3-3-B2 | 3572447- 3574125 | Chr19 | 252 | 4.79 | 39.12 | 28,503.45 | OK491865 |
Mi14-3-3-C1 | 4366341- 4369386 | Chr16 | 265 | 4.80 | 35.13 | 29,899.64 | OK491866 |
Mi14-3-3-C2 | 9746038- 9747732 | Chr9 | 247 | 4.85 | 41.86 | 28,078.73 | OK491867 |
Mi14-3-3-D1 | 18226835- 18229518 | Chr6 | 257 | 4.77 | 46.99 | 29,284.95 | OK491868 |
Mi14-3-3-D2 | 1716202- 1718844 | Chr13 | 257 | 4.68 | 45.77 | 29,422.24 | OK491869 |
Mi14-3-3-E1 | 3882198- 3883306 | Chr19 | 261 | 4.68 | 44.49 | 29,583.16 | OK491870 |
Mi14-3-3-E2 | 4744386- 4745564 | Chr10 | 261 | 4.69 | 51.96 | 29,607.16 | OK491871 |
Mi14-3-3-I1 | 2799060- 2800971 | Chr4 | 278 | 4.83 | 49.60 | 30,968.14 | OK491872 |
Mi14-3-3-I2 | 8264404- 8266523 | Chr8 | 267 | 4.93 | 51.64 | 30,230.90 | OK491873 |
Mi14-3-3-6A | 364937- 367380 | Chr7 | 261 | 4.72 | 48.41 | 29,541.14 | OK203791 |
Mi14-3-3-6B | 12389595- 12391788 | Chr4 | 261 | 4.76 | 50.54 | 29,376.81 | OK203792 |
Mi14-3-3-7A | 4859531- 4862214 | Chr10 | 266 | 5.01 | 42.85 | 30,506.27 | OK491860 |
Mi14-3-3-7B | 4027530- 4010130 | Chr19 | 254 | 4.85 | 45.50 | 28,807.40 | OK491861 |
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Xia, L.; He, X.; Huang, X.; Yu, H.; Lu, T.; Xie, X.; Zeng, X.; Zhu, J.; Luo, C. Genome-Wide Identification and Expression Analysis of the 14-3-3 Gene Family in Mango (Mangifera indica L.). Int. J. Mol. Sci. 2022, 23, 1593. https://doi.org/10.3390/ijms23031593
Xia L, He X, Huang X, Yu H, Lu T, Xie X, Zeng X, Zhu J, Luo C. Genome-Wide Identification and Expression Analysis of the 14-3-3 Gene Family in Mango (Mangifera indica L.). International Journal of Molecular Sciences. 2022; 23(3):1593. https://doi.org/10.3390/ijms23031593
Chicago/Turabian StyleXia, Liming, Xinhua He, Xing Huang, Haixia Yu, Tingting Lu, Xiaojie Xie, Xuemei Zeng, Jiawei Zhu, and Cong Luo. 2022. "Genome-Wide Identification and Expression Analysis of the 14-3-3 Gene Family in Mango (Mangifera indica L.)" International Journal of Molecular Sciences 23, no. 3: 1593. https://doi.org/10.3390/ijms23031593