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Article

Regulation of the Bud Dormancy Development and Release in Micropropagated Rhubarb ‘Malinowy’

1
Department of Applied Biology, Institute of Horticulture—National Research Institute, Konstytucji 3 Maja 1/3 Street, 96-100 Skierniewice, Poland
2
Department of Biotechnology, The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21 Street, 30-239 Kraków, Poland
*
Author to whom correspondence should be addressed.
Academic Editors: Luca Agnelli and Giovanni Malerba
Int. J. Mol. Sci. 2022, 23(3), 1480; https://doi.org/10.3390/ijms23031480
Received: 29 December 2021 / Revised: 19 January 2022 / Accepted: 25 January 2022 / Published: 27 January 2022
(This article belongs to the Special Issue 21st Anniversary of IJMS: Advances in Molecular Genetics and Genomics)
Culinary rhubarb is a vegetable crop, valued for its stalks, very rich in different natural bioactive ingredients. In commercial rhubarb stalk production, the bud dormancy development and release are crucial processes that determine the yields and quality of stalks. To date, reports on rhubarb bud dormancy regulation, however, are lacking. It is known that dormancy status depends on cultivars. The study aimed to determine the dormancy regulation in a valuable selection of rhubarb ‘Malinowy’. Changes in carbohydrate, total phenolic, endogenous hormone levels, and gene expression levels during dormancy development and release were studied in micropropagated rhubarb plantlets. Dormancy developed at high temperature (25.5 °C), and long day. Leaf senescence and dying were consistent with a significant increase in starch, total phenolics, ABA, IAA and SA levels. Five weeks of cooling at 4 °C were sufficient to break dormancy, but rhizomes stored for a longer duration showed faster and more uniformity leaf growing, and higher stalk length. No growth response was observed for non-cooled rhizomes. The low temperature activated carbohydrate and hormone metabolism and signalling in the buds. The increased expression of AMY3, BMY3, SUS3, BGLU17, GAMYB genes were consistent with a decrease in starch and increase in soluble sugars levels during dormancy release. Moreover, some genes (ZEP, ABF2, GASA4, GA2OX8) related to ABA and GA metabolism and signal transduction were activated. The relationship between auxin (IAA, IBA, 5-Cl-IAA), and phenolic, including SA levels and dormancy status was also observed. View Full-Text
Keywords: endogenous hormone; gene expression; phenolics; soluble sugars; starch; temperature endogenous hormone; gene expression; phenolics; soluble sugars; starch; temperature
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MDPI and ACS Style

Wojtania, A.; Markiewicz, M.; Waligórski, P. Regulation of the Bud Dormancy Development and Release in Micropropagated Rhubarb ‘Malinowy’. Int. J. Mol. Sci. 2022, 23, 1480. https://doi.org/10.3390/ijms23031480

AMA Style

Wojtania A, Markiewicz M, Waligórski P. Regulation of the Bud Dormancy Development and Release in Micropropagated Rhubarb ‘Malinowy’. International Journal of Molecular Sciences. 2022; 23(3):1480. https://doi.org/10.3390/ijms23031480

Chicago/Turabian Style

Wojtania, Agnieszka, Monika Markiewicz, and Piotr Waligórski. 2022. "Regulation of the Bud Dormancy Development and Release in Micropropagated Rhubarb ‘Malinowy’" International Journal of Molecular Sciences 23, no. 3: 1480. https://doi.org/10.3390/ijms23031480

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