Bilateral Meningioma: A Case Report and Review of the Literature
Abstract
:1. Introduction
2. Case Description
3. Results
3.1. Sequence Analysis and Deletion/Duplication Testing of NF2 and SMARCB1 Genes
3.2. Levels of E- and N-Cadherins Expression
3.3. Levels of TWIST1, SNAIL and SLUG Expression
3.4. Levels of Beta-Catenin and GSK3beta Expression
3.5. Levels of DVL1 Expression
4. Discussion
5. Methods
5.1. DNA Extraction and Genetic Testing
5.2. Immunohistochemistry
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Meningoma #1 | |
---|---|
Type | Grade I meningothelial meningioma |
Location | Left frontotemporoparietal region |
Consistency and dimensions | Soft consistency with dimensions 7.5 × 6 × 3 cm |
Mitosis | Nuclei of tumor cells were hypochromatic. Only 1 mitosis found in 10 consecutive high-power fields. |
Histological description | Nest configurations of meningothelial cells were placed in pseudosyncytial formation. Minor centers of necrosis were observed. Connective tissue, surrounding the tumor, was partially enlarged. A few psammoma bodies were spotted. On the margins of the specimen, there was a sharp transition from tumor cells to healthy brain tissue. |
Meningoma #2 | |
---|---|
Type | Grade II atypical meningioma |
Location | Right frontotemporoparietal region; tumor attached to dura |
Consistency and dimensions | Grey, solid consistency with dimensions 7 × 4 cm |
Mitosis | Maximum of 5 mitosis in 10 consecutive high-power fields were found |
Histological description | The tumor was built of meningothelial cells forming a pseudosyncytial structure. Hypercellular areas with clear margins between cytoplasmic membranes were spotted. There was no necrosis inside the tissue. Hyalinization and calcification of connective tissue were seen in some areas. A sharp transition from tumor cells to healthy brain parenchyma tissue separated by layers of connective tissue. |
Antigen | Antibody | Type | Dilution |
---|---|---|---|
E-cadherin | E-cadherin clone: NCH-38 Code M3612 (Dako Santa Clara, CA, USA) | Monoclonal | 1:100 |
N-cadherin | N-cadherin (D-4): sc-8424 (Santa Cruz Biotechnology, Inc. Dallas, TX, USA) | Monoclonal | 1:200 |
TWIST1 | Anti-Twist antibody [10E4E6] ab175430 (Abcam Cambridge, MA, USA) | Monoclonal | 1:400 |
SNAIL&SLUG | Anti-SNAIL + SLUG antibody ab180714 (Abcam Cambridge, MA, USA) | Polyclonal | 1:200 |
Beta-catenin (active) | Non-phospho (Active) β-Catenin (Ser33/37/Thr41), (D131A1), (Cell Signalling Technology, Danvers, MA, USA) | Monoclonal | 1:800 |
Beta-catenin (total) | Clone b-Catenin-1, M3539 (Dako, Santa Clara, CA, USA) | Monoclonal | 1:200 |
GSK3beta (active) | Anti-GSK3β (phospho Y216) ab75745 (Abcam, Cambridge, MA, USA) | Polyclonal | 1:100 |
GSK3beta (inactive) | Anti-GSK3β (phospho S9) ab131097 (Abcam, Cambridge, MA, USA) | Polyclonal | 1:100 |
DVL1 | Anti-Dishevelled/Dvl1 antibody: ab233003 (Abcam Cambridge, MA, USA) | Polyclonal | 1:200 |
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Bukovac, A.; Panić, H.; Mrgan, T.; Šlaus, N.; Kafka, A.; Njirić, N.; Pećina-Šlaus, N. Bilateral Meningioma: A Case Report and Review of the Literature. Int. J. Mol. Sci. 2022, 23, 1187. https://doi.org/10.3390/ijms23031187
Bukovac A, Panić H, Mrgan T, Šlaus N, Kafka A, Njirić N, Pećina-Šlaus N. Bilateral Meningioma: A Case Report and Review of the Literature. International Journal of Molecular Sciences. 2022; 23(3):1187. https://doi.org/10.3390/ijms23031187
Chicago/Turabian StyleBukovac, Anja, Hana Panić, Tomislava Mrgan, Nika Šlaus, Anja Kafka, Niko Njirić, and Nives Pećina-Šlaus. 2022. "Bilateral Meningioma: A Case Report and Review of the Literature" International Journal of Molecular Sciences 23, no. 3: 1187. https://doi.org/10.3390/ijms23031187