Genome-Wide Analysis of Circular RNAs Reveals circCHRNG Regulates Sheep Myoblast Proliferation via miR-133/SRF and MEF2A Axis
Abstract
:1. Introduction
2. Results
2.1. Identification of circRNAs during Sheep Skeletal Muscle Development
2.2. Identification of DEcircRNAs
2.3. Functional Enrichment Analysis of DEcircRNAs
2.4. Trend Analysis of circRNAs during Muscle Development
2.5. CircCHRNG Promote Myoblast Proliferation
2.6. CircCHRNG interacts with miR-133 and Regulates the Expression of SRF and MEF2A in Myoblasts
2.7. CircCHRNG Functions as a Sponge of miR-133 to Promote Myoblast Proliferation
3. Discussion
4. Materials and Methods
4.1. Experimental Samples and Ethics Statement
4.2. Library Construction and Sequencing
4.3. Identification of Circular RNAs in Sheep Muscle
4.4. Differential Expression Analysis
4.5. Target Site Prediction and Functional Enrichment Analysis
4.6. Data Validation
4.7. Cell Culture and Transfection
4.8. Dual-Luciferase Reporter Assay
4.9. Cell Proliferation Assays
4.10. Western Blot Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, Y.; Chen, Q.; Bao, J.; Pu, Y.; Han, J.; Zhao, H.; Ma, Y.; Zhao, Q. Genome-Wide Analysis of Circular RNAs Reveals circCHRNG Regulates Sheep Myoblast Proliferation via miR-133/SRF and MEF2A Axis. Int. J. Mol. Sci. 2022, 23, 16065. https://doi.org/10.3390/ijms232416065
Liu Y, Chen Q, Bao J, Pu Y, Han J, Zhao H, Ma Y, Zhao Q. Genome-Wide Analysis of Circular RNAs Reveals circCHRNG Regulates Sheep Myoblast Proliferation via miR-133/SRF and MEF2A Axis. International Journal of Molecular Sciences. 2022; 23(24):16065. https://doi.org/10.3390/ijms232416065
Chicago/Turabian StyleLiu, Yue, Qian Chen, Jingjing Bao, Yabin Pu, Jianlin Han, Huijing Zhao, Yuehui Ma, and Qianjun Zhao. 2022. "Genome-Wide Analysis of Circular RNAs Reveals circCHRNG Regulates Sheep Myoblast Proliferation via miR-133/SRF and MEF2A Axis" International Journal of Molecular Sciences 23, no. 24: 16065. https://doi.org/10.3390/ijms232416065