Influence of Single-Nucleotide Polymorphisms on Vitamin D Receptor Expression in Periodontal Ligament Fibroblasts as a Response to Orthodontic Compression
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Ethics Statement
4.2. Sample Collection and In Vitro Compression Model for Orthodontic Tooth Movement
4.3. Total RNA Isolation and Quantification of Relative Gene Expression (RT-qPCR)
4.4. DNA Extraction and Genotyping Analysis
4.5. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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SNP | Reference Sequence | # Base Change | # Gene Function and Location | * Genotyping Rate |
---|---|---|---|---|
BgII | rs739837 | G/T | 3 Prime UTR Variant | 54/57 (94.6%) |
BsmI | rs1544410 | T/C | Intron Variant | 55/57 (96.5%) |
Apal | rs7975232 | A/C | Intron Variant | 57/57 (100%) |
FokI | rs2228570 | A/G | Missense Variant | 57/57 (100%) |
TaqI | rs731236 | A/G | Synonymous Variant | 53/57 (92.9%) |
SNP | Genotype | n | Fold Change VDR Expression | p-Value | ||
---|---|---|---|---|---|---|
Median | 25th Percentile | 75th Percentile | ||||
BgII | GG | 12 | 0.78 | 0.36 | 1.18 | 0.874 |
GT | 27 | 0.74 | 0.52 | 1.16 | ||
TT | 15 | 0.95 | 0.48 | 1.65 | ||
BsmI | CC | 17 | 0.97 | 0.36 | 1.61 | 0.579 |
CT | 34 | 0.68 | 0.54 | 1.12 | ||
TT | 5 | 1.01 | 0.94 | 1.41 | ||
Apal | AA | 14 | 0.84 | 0.54 | 1.42 | 0.844 |
AC | 27 | 0.87 | 0.43 | 1.62 | ||
CC | 16 | 0.65 | 0.45 | 1.17 | ||
FokI | AA | 8 | 0.69 | 0.57 | 1.18 | 0.938 |
AG | 25 | 0.87 | 0.41 | 1.22 | ||
GG | 24 | 0.89 | 0.50 | 1.20 | ||
TaqI | AA | 4 | 0.87 | 0.66 | 1.30 | 0.901 |
AG | 39 | 0.80 | 0.48 | 1.97 | ||
GG | 12 | 0.71 | 0.42 | 1.22 |
Variable | Reference * | Estimate | Standard Error | 95% CI | t |
---|---|---|---|---|---|
BgII [GT] | GG | −1.33 | 1.50 | −4.37 to 1.71 | 0.88 |
BgII [TT] | −1.40 | 1.71 | −4.85 to 2.05 | 0.81 | |
BsmI [CT] | CC | −1.32 | 1.09 | −3.54 to 0.89 | 1.20 |
BsmI [TT] | 0.36 | 2.05 | −3.77 to 4.51 | 0.17 | |
Apal [AC] | CC | 0.18 | 1.25 | −2.36 to 2.72 | 0.14 |
Apal [AA] | 0.56 | 1.66 | −2.79 to 3.92 | 0.33 | |
FokI [AA] | AG | 0.01 | 1.27 | −2.55 to 2.59 | 0.01 |
FokI [GG] | 0.67 | 1.03 | −1.41 to 2.76 | 0.65 | |
TaqI [GG] | AA | 0.19 | 2.30 | −4.45 to 4.84 | 0.08 |
TaqI [AG] | 1.55 | 2.10 | −2.70 to 5.80 | 0.73 |
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Küchler, E.C.; Schröder, A.; Spanier, G.; Thedei, G., Jr.; Carvalho Ribeiro de Oliveira, M.B.; de Menezes-Oliveira, M.A.H.; Proff, P.; Kirschneck, C. Influence of Single-Nucleotide Polymorphisms on Vitamin D Receptor Expression in Periodontal Ligament Fibroblasts as a Response to Orthodontic Compression. Int. J. Mol. Sci. 2022, 23, 15948. https://doi.org/10.3390/ijms232415948
Küchler EC, Schröder A, Spanier G, Thedei G Jr., Carvalho Ribeiro de Oliveira MB, de Menezes-Oliveira MAH, Proff P, Kirschneck C. Influence of Single-Nucleotide Polymorphisms on Vitamin D Receptor Expression in Periodontal Ligament Fibroblasts as a Response to Orthodontic Compression. International Journal of Molecular Sciences. 2022; 23(24):15948. https://doi.org/10.3390/ijms232415948
Chicago/Turabian StyleKüchler, Erika Calvano, Agnes Schröder, Gerrit Spanier, Geraldo Thedei, Jr., Maria Beatriz Carvalho Ribeiro de Oliveira, Maria Angélica Hueb de Menezes-Oliveira, Peter Proff, and Christian Kirschneck. 2022. "Influence of Single-Nucleotide Polymorphisms on Vitamin D Receptor Expression in Periodontal Ligament Fibroblasts as a Response to Orthodontic Compression" International Journal of Molecular Sciences 23, no. 24: 15948. https://doi.org/10.3390/ijms232415948