Klotho Protein Decreases MMP-Mediated Degradation of Contractile Proteins during Ischaemia/Reperfusion Injury to the Cardiomyocytes
Abstract
:1. Introduction
2. Results
2.1. The Level of Injury to Cardiac Cells
2.2. Expression of MMP Genes in Human Myocytes
2.3. Expression of the MMP Proteins in Human Myocytes
2.4. Release of Contractile Proteins from Cardiac Myocytes
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. In Vitro Chemical Ischaemia/Reperfusion Injury to Human Cardiomyocytes
4.3. LDH Activity Measurement
4.4. MMP-2 and MMP-9 mRNA Expression
4.5. Immunofluorescence Staining for MMP-2 and MMP-9 Proteins
4.6. Analysis of MMP-2 Concentration
4.7. Zymography
4.8. The Measurement of Contractile Proteins Release
4.9. Determining the Total Protein Concentration
4.10. Statistical Analysis
5. Conclusions
Limitations
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Olejnik, A.; Krzywonos-Zawadzka, A.; Banaszkiewicz, M.; Bil-Lula, I. Klotho Protein Decreases MMP-Mediated Degradation of Contractile Proteins during Ischaemia/Reperfusion Injury to the Cardiomyocytes. Int. J. Mol. Sci. 2022, 23, 15450. https://doi.org/10.3390/ijms232415450
Olejnik A, Krzywonos-Zawadzka A, Banaszkiewicz M, Bil-Lula I. Klotho Protein Decreases MMP-Mediated Degradation of Contractile Proteins during Ischaemia/Reperfusion Injury to the Cardiomyocytes. International Journal of Molecular Sciences. 2022; 23(24):15450. https://doi.org/10.3390/ijms232415450
Chicago/Turabian StyleOlejnik, Agnieszka, Anna Krzywonos-Zawadzka, Marta Banaszkiewicz, and Iwona Bil-Lula. 2022. "Klotho Protein Decreases MMP-Mediated Degradation of Contractile Proteins during Ischaemia/Reperfusion Injury to the Cardiomyocytes" International Journal of Molecular Sciences 23, no. 24: 15450. https://doi.org/10.3390/ijms232415450