Branched Poly(ε-caprolactone)-Based Copolyesters of Different Architectures and Their Use in the Preparation of Anticancer Drug-Loaded Nanoparticles
Abstract
:1. Introduction
2. Results and Discussion
2.1. Branched PCL/Polyol Copolymers Synthesis
2.1.1. H NMR Analysis
2.1.2. FTIR Analysis
2.2. Copolymer Characterization
2.2.1. Molecular Weight and Intrinsic Viscosity
2.2.2. Physical State and Thermal Properties Evaluation
2.2.3. Enzymatic Hydrolysis
2.2.4. Cytotoxicity Evaluation
2.3. PTX-Loaded NPs Characterization
2.3.1. NPs’ Morphology and Size
2.3.2. PTX Physical State Evaluation
2.3.3. PTX-Copolymer Molecular Interactions
2.3.4. Drug Loading, Encapsulation Efficiency (EE), and Yield
2.3.5. In Vitro Drug Dissolution Studies
2.3.6. Modeling of Release Kinetics
3. Materials and Methods
3.1. Materials
3.2. Preparation of the Synthesized Branched PCL Copolymers
3.3. Characterization of the Synthesized Branched PCL Copolymers
3.3.1. Nuclear Magnetic Resonance Spectroscopy
3.3.2. Intrinsic Viscosity
3.3.3. Powder X-Ray (pXRD) Diffractometry
3.3.4. Fourier-Transform Infrared (FTIR) Spectroscopy
3.3.5. Differential Scanning Calorimetry (DSC)
3.3.6. Size Exclusion Chromatography (SEC)
3.3.7. Enzymatic Hydrolysis
3.3.8. Cytotoxicity Studies
3.4. Preparation of NPs
3.5. Characterization of NPs
3.5.1. Physical State Evaluation
3.5.2. Evaluation of Molecular Interactions
3.5.3. Evaluation of Particle Size Distribution (PSD) and NPs’ Morphology
3.5.4. Drug Loading, Encapsulation Efficiency (EE), and Yield
3.5.5. In Vitro Dissolution Studies
3.5.6. HPLC Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Sample | Mn (g/mol) | Mw (g/mol) | PDI |
---|---|---|---|
PCL-GLY | 43,300 | 60,400 | 1.39 |
PCL-PE | 29,000 | 32,900 | 1.13 |
PCL-XYL | 21,116 | 26,438 | 1.25 |
Sample | Yield (%) | Drug Loading (%) | EE (%) |
---|---|---|---|
NPs(PCL-GLY + PTΧ) | 96 ± 1.38 | 5.97 ± 1.39 | 60.09 ± 2.02 |
NPs(PCL-PE + PTΧ) | 90 ± 2.56 | 6.58 ± 2.02 | 66.26 ± 2.85 |
NPs(PCL-XYL + PTΧ) | 86 ± 1.84 | 7.42 ± 1.98 | 72.01 ± 2.70 |
Sample | φ1 | φ2 | K1 (h−1) | K2 (h−1) | K3 (h−1) |
---|---|---|---|---|---|
PCL-GLY | 14.1 | 23.9 | 1.95 | 0.043 | 0.064 |
PCL-PE | 29 | 26.4 | 4.65 | 0.066 | 0.106 |
PCL-XYL | 47.7 | 11.9 | 1.68 | 0.072 | 0.05 |
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Christodoulou, E.; Notopoulou, M.; Nakiou, E.; Kostoglou, M.; Barmpalexis, P.; Bikiaris, D.N. Branched Poly(ε-caprolactone)-Based Copolyesters of Different Architectures and Their Use in the Preparation of Anticancer Drug-Loaded Nanoparticles. Int. J. Mol. Sci. 2022, 23, 15393. https://doi.org/10.3390/ijms232315393
Christodoulou E, Notopoulou M, Nakiou E, Kostoglou M, Barmpalexis P, Bikiaris DN. Branched Poly(ε-caprolactone)-Based Copolyesters of Different Architectures and Their Use in the Preparation of Anticancer Drug-Loaded Nanoparticles. International Journal of Molecular Sciences. 2022; 23(23):15393. https://doi.org/10.3390/ijms232315393
Chicago/Turabian StyleChristodoulou, Evi, Maria Notopoulou, Eirini Nakiou, Margaritis Kostoglou, Panagiotis Barmpalexis, and Dimitrios N. Bikiaris. 2022. "Branched Poly(ε-caprolactone)-Based Copolyesters of Different Architectures and Their Use in the Preparation of Anticancer Drug-Loaded Nanoparticles" International Journal of Molecular Sciences 23, no. 23: 15393. https://doi.org/10.3390/ijms232315393