Structural and Immunologic Properties of the Major Soybean Allergen Gly m 4 Causing Anaphylaxis
Abstract
:1. Introduction
2. Results and Discussion
2.1. Investigation of Immunological Similarity of the Birch Pollen Bet v 1 and the Soybean Gly m 4 by ELISA
2.2. Effects of Heating on the Gly m 4 Structure and IgE-Binding Capacity
2.3. Effects of Different Gastric pH Values and Pepsin Loadings on Proteolysis of Gly m 4 Mimicking Gastroduodenal Digestion of the Allergen and Its IgE-Binding Capacity
2.4. Preparation and Characterization of Microsomal or Lysosomal Fractions of Human Macrophages
2.5. Comparison of the Birch Bet v 1 and the Soybean Gly m 4 Endolysosomal Degradation
3. Materials and Methods
3.1. Materials
3.2. CD Spectroscopy
3.3. Gastroduodenal Digestion In Vitro
3.4. Antibodies Preparation
3.5. Immunoglobulin Binding Assay
3.6. THP-1 Cell Culture and Macrophage Differentiation
3.7. Subcellular Fractionation of Microsomes and Lysosomes
3.8. Assay for Determination of Marker Lysosomal Enzyme N-acetyl-β-d-glucosaminidase
3.9. Allergen Degradation by the Content of Microsomal and Lysosomal Fractions
3.10. LC-MS/MS
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Peptide | Amino Acid Residues | −10lgP | Molecular Mass | m/z | Peak Area |
---|---|---|---|---|---|
VAPQAISSVENIEGNGGPGTIKK | 33–55 | 50.85 | 2265.2 | 1133.6 | 1.35 × 1010 |
IVATPDGGSILK | 104–115 | 36.56 | 1169.7 | 585.8 | 1.34 × 1010 |
ISNKYHTKGDHEVK | 116–129 | 48.99 | 1654.8 | 828.4 | 1.01 × 1010 |
VAPQAISSVENIEGNGGPGTIK | 33–54 | 53.79 | 2137.1 | 1069.6 | 9.34 × 109 |
DRVDEVDHTNFK | 69–80 | 44.56 | 1473.7 | 737.9 | 8.62 × 109 |
SVIEGGPIGDTLEK | 84–97 | 37.60 | 1413.7 | 707.9 | 8.05 × 109 |
YHTKGDHEVK | 120–129 | 37.56 | 1212.6 | 607.3 | 5.13 × 109 |
IEGGPIGDTLEK | 86–97 | 40.35 | 1227.6 | 614.8 | 4.50 × 109 |
TPDGGSILK | 107–115 | 24.76 | 886.5 | 444.2 | 3.52 × 109 |
NYETETTSVIPAAR | 4–17 | 40.63 | 1550.8 | 776.4 | 2.63 × 109 |
VIEGGPIGDTLEK | 85–97 | 40.75 | 1326.7 | 664.4 | 2.09 × 109 |
NKYHTKGDHEVK | 118–129 | 44.99 | 1454.7 | 728.4 | 1.65 × 109 |
KISFPEGFPFK | 55–65 | 39.27 | 1295.7 | 648.9 | 1.49 × 109 |
VAPQAISSVENIEGN(+0.98)GGPGTIKK * | 33–55 | 42.90 | 2266.2 | 1134.1 | 1.37 × 109 |
AISSVENIEGNGGPGTIK | 37–54 | 43.50 | 1741.9 | 871.9 | 1.09 × 109 |
Peptide | Amino Acid Residues | −10lgP | Molecular Mass | m/z | Peak Area |
---|---|---|---|---|---|
SVENVEGNGGPGTIKK | 39–54 | 46.07 | 1584.8 | 793.4 | 1.91 × 1010 |
SVENVEGN(+0.98)GGPGTIKK * | 39–54 | 44.67 | 1585.8 | 397.5 | 1.17 × 1010 |
KSVENVEGN(+0.98)GGPGTIK * | 38–53 | 19.77 | 1585.8 | 529.6 | 7.07 × 109 |
VVGGAALPDTAEK | 84–96 | 44.67 | 1226.7 | 614.3 | 6.39 × 109 |
SVEN(+0.98)VEGNGGPGTIKK * | 39–54 | 33.12 | 1585.8 | 793.9 | 4.63 × 109 |
ENVEGNGGPGTIKK | 41–54 | 40.12 | 1398.7 | 467.2 | 3.00 × 109 |
NVEGNGGPGTIK | 42–53 | 29.76 | 1141.6 | 571.8 | 2.01 × 109 |
IESIDEANLGYSYSVVGGAALPDTAEK | 70–96 | 59.08 | 2768.3 | 1385.2 | 1.75 × 109 |
NVEGN(+0.98)GGPGTIKK * | 42–54 | 37.41 | 1270.7 | 424.6 | 1.64 × 109 |
ETKGDAEPNQDELK | 120–133 | 51.28 | 1572.7 | 787.4 | 1.51 × 109 |
TKGDAEPNQDELK | 121–133 | 50.34 | 1443.7 | 722.9 | 1.49 × 109 |
IDEANLGYSYSVVGGAALPDTAEK | 73–96 | 55.12 | 2439.2 | 1220.6 | 1.22 × 109 |
YETKGDAEPNQDELK | 119–133 | 51.19 | 1735.8 | 868.9 | 1.21 × 109 |
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Finkina, E.I.; Bogdanov, I.V.; Ziganshin, R.H.; Strokach, N.N.; Melnikova, D.N.; Toropygin, I.Y.; Matveevskaya, N.S.; Ovchinnikova, T.V. Structural and Immunologic Properties of the Major Soybean Allergen Gly m 4 Causing Anaphylaxis. Int. J. Mol. Sci. 2022, 23, 15386. https://doi.org/10.3390/ijms232315386
Finkina EI, Bogdanov IV, Ziganshin RH, Strokach NN, Melnikova DN, Toropygin IY, Matveevskaya NS, Ovchinnikova TV. Structural and Immunologic Properties of the Major Soybean Allergen Gly m 4 Causing Anaphylaxis. International Journal of Molecular Sciences. 2022; 23(23):15386. https://doi.org/10.3390/ijms232315386
Chicago/Turabian StyleFinkina, Ekaterina I., Ivan V. Bogdanov, Rustam H. Ziganshin, Nikita N. Strokach, Daria N. Melnikova, Ilia Y. Toropygin, Natalia S. Matveevskaya, and Tatiana V. Ovchinnikova. 2022. "Structural and Immunologic Properties of the Major Soybean Allergen Gly m 4 Causing Anaphylaxis" International Journal of Molecular Sciences 23, no. 23: 15386. https://doi.org/10.3390/ijms232315386