Correlation between Adrenoceptor Expression and Clinical Parameters in Degenerated Lumbar Intervertebral Discs
Abstract
:1. Introduction
2. Results
2.1. Patient Characteristics
2.2. AR Gene Expression and Modic Classification
2.3. AR Gene Expression and Pfirrmann Classification
2.4. AR Gene Expression and Clinical Parameters
2.5. Adjacent Segment Disease
3. Discussion
4. Materials and Methods
4.1. Human IVD Tissue Samples and Patient Characteristics
4.2. AR Gene Expression
4.3. Radiological Classification of IVDD and Patient Characteristics
4.4. Ethical Approval
4.5. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Patient Characteristics | |
---|---|
Patients, n | 43 |
Sex, no. female (%)/no. male (%) | 34 (79.1%)/9 (20.9%) |
Age [years], mean (SD) | 69.23 (7.66) |
BMI [kg/m2], mean (SD) | 30.77 (5.67) |
Preoperative NPRS, mean (SD) | 6.81 (1.53) |
Postoperative NPRS, mean (SD) | 2.40 (1.61) |
Adjacent segment disease, n (%) | 7 (16.3%) |
Nicotine abuse, n (%) | 11 (25.6%) |
Medication: NSAID, n (%) | 20 (46.5%) |
Medication: β-blocker, n (%) | 22 (51.2%) |
Level of operation: | |
L 1/2 | 2 (4.7%) |
L 2/3 | 6 (14.0%) |
L 3/4 | 9 (20.9%) |
L 4/5 | 15 (34.9%) |
L5/S1 | 11 (25.6%) |
Total | 43 (100%) |
Modic classification, n (%) | |
0 | 10 (23.3%) |
I | 9 (20.9%) |
II | 20 (46.5%) |
III | 3 (7.0%) |
Missing MRI | 1 (2.3%) |
Total | 43 (100%) |
Pfirrmann classification, n (%) | |
I | 0 (0%) |
II | 2 (4.7%) |
III | 13 (30.2%) |
IV | 14 (32.6%) |
V | 13 (30.2%) |
Missing MRI | 1 (2.3%) |
Total | 43 (100%) |
Gene Symbol | NCBI Reference | Forward (5′-3′) | Reverse (5′-3′) |
---|---|---|---|
GAPDH | NM_001289745.2 | CTCCTGTTCGACAGTCAGCC | TTCCCGTTCTCAGCCTTGAC |
ADRA1A | NM_000680.3 | CCATGCTCCAGCCAAGAGTT | TCCTGTCCTAGACTTCCTCCC |
ADRA1B | NM_000679.3 | GTCCACCGTCATCTCCATCG | GAACAAGGAGCCAAGCGGTAG |
ADRA1D | NM_000678.3 | TGACTTTCCGCGATCTCCTG | TTACCTGCCACGGCCATAAG |
ADRA2A | NM_000681.3 | TGGTCATCGGAGTGTTCGTG | GCCCACTAGGAAGATGGCTC |
ADRA2B | NM_000682.6 | GACATTTCACCGGCAACACC | GGGACTGAGAACCAGGAAGC |
ADRA2C | NM000683.3 | CGATGTGCTGTTTTGCACCT | GGATGTACCAGGTCTCGTCG |
ADRB1 | NM_000684.2 | TAGCAGGTGAACTCGAAGCC | ATCTTCCACTCCGGTCCTCT |
ADRB2 | NM_000024.5 | CAGAGCCTGCTGACCAAGAA | GCCTAACGTCTTGAGGGCTT |
ADRB3 | NM_000025.2 | GCCAATTCTGCCTTCAACCC | GCCAGAGGTTTTCCACAGGT |
TH | NM_000360.3 | CAGGCAGAGGCCATCATGT | GTGGTCCAAGTCCAGGTCAG |
Modic Classification | ||||
---|---|---|---|---|
Vertebral Endplates | Histopathology | Signal intensity | ||
Modic I | Marrow edema | T1 hypointense, T2 hyperintense | ||
Modic II | Fatty degeneration | T1 hyperintense, T2 iso-/hyperintense | ||
Modic III | Subchondral bony sclerosis | T1 hypointense, T2 hypointense | ||
Pfirrmann classification | ||||
Grade | Structure | Signal intensity (T2) | Distinction of Nucleus and Anulus | Height |
Pfirrmann I | homogeneous, white | hyperintense | clear | normal |
Pfirrmann II | inhomogeneous, horizontal bands | hyperintense | clear | normal |
Pfirrmann III | inhomogeneous, gray | intermediate | unclear | normal– slightly decreased |
Pfirrmann IV | inhomogeneous, gray to black | intermediate to hypointense | lost | slightly– moderately decreased |
Pfirrmann V | inhomogeneous, black | hypointense | lost | collapsed |
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Brenneis, M.; Jenei-Lanzl, Z.; Kupka, J.; Braun, S.; Junker, M.; Zaucke, F.; Rickert, M.; Meurer, A. Correlation between Adrenoceptor Expression and Clinical Parameters in Degenerated Lumbar Intervertebral Discs. Int. J. Mol. Sci. 2022, 23, 15358. https://doi.org/10.3390/ijms232315358
Brenneis M, Jenei-Lanzl Z, Kupka J, Braun S, Junker M, Zaucke F, Rickert M, Meurer A. Correlation between Adrenoceptor Expression and Clinical Parameters in Degenerated Lumbar Intervertebral Discs. International Journal of Molecular Sciences. 2022; 23(23):15358. https://doi.org/10.3390/ijms232315358
Chicago/Turabian StyleBrenneis, Marco, Zsuzsa Jenei-Lanzl, Johannes Kupka, Sebastian Braun, Marius Junker, Frank Zaucke, Marcus Rickert, and Andrea Meurer. 2022. "Correlation between Adrenoceptor Expression and Clinical Parameters in Degenerated Lumbar Intervertebral Discs" International Journal of Molecular Sciences 23, no. 23: 15358. https://doi.org/10.3390/ijms232315358