Role of Acrostyle Cuticular Proteins in the Retention of an Aphid Salivary Effector
Abstract
:1. Introduction
2. Results
2.1. Stylin-03 Interacts with the Effector Mp10 in Yeast
2.2. Mp10 Binds to the Acrostyle of A. Pisum and M. Persicae
2.3. Impact of siRNA Targeting Stylin-03 or Stylin-01 Gene Expression on M. Persicae Feeding Behavior
2.4. Impact of siRNA Targeting Stylin-03 or Stylin-01 Gene Expression on M. Persicae Survival Rate and Fecundity
3. Discussion
4. Materials and Methods
4.1. Aphid Colonies
4.2. Yeast Two-Hybrid Constructs and Assays
4.3. Constructs, Production and Labeling of Mp10 for Detection on Insect Stylets
4.4. In Vitro Interactions between Mp10 and Dissected Aphid Stylets
4.5. RNA Interference
4.6. Quantitative PCR
4.7. Analysis of Aphid Feeding Behavior
4.8. Aphid Life Table Statistics
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Deshoux, M.; Monsion, B.; Pichon, E.; Jiménez, J.; Moreno, A.; Cayrol, B.; Thébaud, G.; Mugford, S.T.; Hogenhout, S.A.; Blanc, S.; et al. Role of Acrostyle Cuticular Proteins in the Retention of an Aphid Salivary Effector. Int. J. Mol. Sci. 2022, 23, 15337. https://doi.org/10.3390/ijms232315337
Deshoux M, Monsion B, Pichon E, Jiménez J, Moreno A, Cayrol B, Thébaud G, Mugford ST, Hogenhout SA, Blanc S, et al. Role of Acrostyle Cuticular Proteins in the Retention of an Aphid Salivary Effector. International Journal of Molecular Sciences. 2022; 23(23):15337. https://doi.org/10.3390/ijms232315337
Chicago/Turabian StyleDeshoux, Maëlle, Baptiste Monsion, Elodie Pichon, Jaime Jiménez, Aránzazu Moreno, Bastien Cayrol, Gaël Thébaud, Sam T. Mugford, Saskia A. Hogenhout, Stéphane Blanc, and et al. 2022. "Role of Acrostyle Cuticular Proteins in the Retention of an Aphid Salivary Effector" International Journal of Molecular Sciences 23, no. 23: 15337. https://doi.org/10.3390/ijms232315337