CRISPR/Cas9 Gene Editing of NtAITRs, a Family of Transcription Repressor Genes, Leads to Enhanced Drought Tolerance in Tobacco
Abstract
:1. Introduction
2. Results
2.1. Identification of AITRs in Tobacco
2.2. Expression Levels of NtAITRs Are Increased in Response to ABA and Drought Treatments
2.3. Generation of Transgene-Free Ntaitr Mutants
2.4. The ntaitr12356 Mutant Plants Show Enhanced Tolerance to Drought
2.5. The ntatir12356 Mutants Are Hypersensitive to ABA
3. Discussion
4. Materials and Methods
4.1. Identification of AITRs in Tobacco
4.2. Plant Materials and Growth Conditions
4.3. ABA and Drought Treatment, RNA Isolation and RT-PCR
4.4. Constructs
4.5. Plasmid Isolation, Protoplast Isolation and Transient Transfection
4.6. Generation of Transgene-Free ntaitr Mutants
4.7. Seed Germination and Seedling Greening Assays
4.8. Drought Tolerance Assays
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Li, G.; Ma, Y.; Wang, X.; Cheng, N.; Meng, D.; Chen, S.; Wang, W.; Wang, X.; Hu, X.; Yan, L.; et al. CRISPR/Cas9 Gene Editing of NtAITRs, a Family of Transcription Repressor Genes, Leads to Enhanced Drought Tolerance in Tobacco. Int. J. Mol. Sci. 2022, 23, 15268. https://doi.org/10.3390/ijms232315268
Li G, Ma Y, Wang X, Cheng N, Meng D, Chen S, Wang W, Wang X, Hu X, Yan L, et al. CRISPR/Cas9 Gene Editing of NtAITRs, a Family of Transcription Repressor Genes, Leads to Enhanced Drought Tolerance in Tobacco. International Journal of Molecular Sciences. 2022; 23(23):15268. https://doi.org/10.3390/ijms232315268
Chicago/Turabian StyleLi, Guimin, Yanxing Ma, Xiaoping Wang, Nini Cheng, Deyu Meng, Siyu Chen, Wei Wang, Xutong Wang, Xiaojun Hu, Li Yan, and et al. 2022. "CRISPR/Cas9 Gene Editing of NtAITRs, a Family of Transcription Repressor Genes, Leads to Enhanced Drought Tolerance in Tobacco" International Journal of Molecular Sciences 23, no. 23: 15268. https://doi.org/10.3390/ijms232315268