Mulberry Leaf Supplements Effecting Anti-Inflammatory Genes and Improving Obesity in Elderly Overweight Dogs
Abstract
:1. Introduction
2. Results
2.1. Body Condition Score (BCS) and Body Weight (BW)
2.2. Obesity-Related Factors in the Blood
2.3. Preprocessing and Alignment of Reads
2.4. Assembly and Expression Analysis
2.5. Functional Enrichment Analysis and Obesity-Related Genes
2.6. Real-Time PCR Analysis for Validation
2.7. Gut Microbiome and Diversity Analysis
2.8. Taxonomy Analysis of the Samples
2.9. Correlation Analysis between Obesity-Related Factors and Gut Microbiome
2.10. Functional Potential of Bacterial Community
3. Discussion
4. Materials and Methods
4.1. Animals and Diet
4.2. Blood Sampling, Serum Chemistry, and Fecal Sampling
4.3. RNA-Seq Pipeline Used for Assembly and Differential Expression Analysis
4.4. Comparison of DEGs in Different Groups
4.5. Functional Enrichment of DEGs and Identification of Obesity-Related Genes
4.6. Blood Gene Expression Using Real-Time PCR Analysis
4.7. Gut Microbiome Sequence Analysis
4.8. Taxonomic Annotation
4.9. Diversity Analysis
4.10. Differential Abundance of Taxa
4.11. Correlation Analysis between Obesity-Related Factors and Gut Microbiome
4.12. Prediction of Functional Potential of Bacterial Community with Amplicon Sequences
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Group | Species | Gender | Age (y) | BW (kg) | BCS (9-Scale) |
---|---|---|---|---|---|
Mulberry_0 | Cocker Spaniels | F | 7 | 11.5 | 8 |
Beagle | F | 9 | 16.5 | 9 | |
Cocker Spaniels | F | 10 | 17.4 | 9 | |
Cocker Spaniels | M | 10 | 12.1 | 8 | |
Shetland Sheepdog | F | 6 | 11.5 | 7 | |
Mulberry_40 | Beagle | M | 8 | 15.8 | 9 |
Cocker Spaniels | M | 6 | 11.7 | 8 | |
Cocker Spaniels | F | 9 | 10.0 | 7 | |
Cocker Spaniels | M | 9 | 12.9 | 8 | |
Shetland Sheepdog | F | 7 | 11.8 | 7 | |
Mulberry_100 | Beagle | F | 9 | 17.9 | 9 |
Cocker Spaniels | M | 8 | 12.2 | 8 | |
Shetland Sheepdog | F | 6 | 10.8 | 7 | |
Cocker Spaniels | F | 8 | 9.0 | 7 | |
Cocker Spaniels | F | 9 | 11.5 | 7 |
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Park, M.; Jaiswal, V.; Kim, K.; Chun, J.; Lee, M.-J.; Shin, J.-H.; Lee, H.-J. Mulberry Leaf Supplements Effecting Anti-Inflammatory Genes and Improving Obesity in Elderly Overweight Dogs. Int. J. Mol. Sci. 2022, 23, 15215. https://doi.org/10.3390/ijms232315215
Park M, Jaiswal V, Kim K, Chun J, Lee M-J, Shin J-H, Lee H-J. Mulberry Leaf Supplements Effecting Anti-Inflammatory Genes and Improving Obesity in Elderly Overweight Dogs. International Journal of Molecular Sciences. 2022; 23(23):15215. https://doi.org/10.3390/ijms232315215
Chicago/Turabian StylePark, Miey, Varun Jaiswal, Kihyun Kim, Julan Chun, Mi-Jin Lee, Jae-Ho Shin, and Hae-Jeung Lee. 2022. "Mulberry Leaf Supplements Effecting Anti-Inflammatory Genes and Improving Obesity in Elderly Overweight Dogs" International Journal of Molecular Sciences 23, no. 23: 15215. https://doi.org/10.3390/ijms232315215