CD200 as a Potential New Player in Inflammation during Rotator Cuff Tendon Injury/Repair: An In Vitro Model
Abstract
:1. Introduction
2. Results
2.1. Identification of Cell Surface Markers Characterizing the TSPC Population
2.2. CD146+TSPCs Response to TNFα and IFNγ
2.3. TNFα and IFNγ Increased the Expression of CD146+CD200+ TSPCs
2.4. The In Vitro Gap Repair Assay
2.5. IFNγ+TNFα Increased the Expression of STAT1, STAT3, and MMP9 Proteins
2.6. IFNγ+TNFα Increased the Expression of IRF1, CAAT and DOK2 mRNA
3. Discussion
4. Materials and Methods
4.1. Rotator Cuff Tendon-Derived Cells Cultures
4.2. Flow Cytometry
4.3. In Vitro Gap Repair Assay
4.4. Immunoblotting
4.5. Gene Expression Analysis
4.6. Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Giancola, R.; Oliva, F.; Gallorini, M.; Michetti, N.; Gissi, C.; Moussa, F.; Antonetti Lamorgese Passeri, C.; Colosimo, A.; Berardi, A.C. CD200 as a Potential New Player in Inflammation during Rotator Cuff Tendon Injury/Repair: An In Vitro Model. Int. J. Mol. Sci. 2022, 23, 15165. https://doi.org/10.3390/ijms232315165
Giancola R, Oliva F, Gallorini M, Michetti N, Gissi C, Moussa F, Antonetti Lamorgese Passeri C, Colosimo A, Berardi AC. CD200 as a Potential New Player in Inflammation during Rotator Cuff Tendon Injury/Repair: An In Vitro Model. International Journal of Molecular Sciences. 2022; 23(23):15165. https://doi.org/10.3390/ijms232315165
Chicago/Turabian StyleGiancola, Raffaella, Francesco Oliva, Marialucia Gallorini, Noemi Michetti, Clarissa Gissi, Fadl Moussa, Cristina Antonetti Lamorgese Passeri, Alessia Colosimo, and Anna Concetta Berardi. 2022. "CD200 as a Potential New Player in Inflammation during Rotator Cuff Tendon Injury/Repair: An In Vitro Model" International Journal of Molecular Sciences 23, no. 23: 15165. https://doi.org/10.3390/ijms232315165