TRPV4 Promotes Metastasis in Melanoma by Regulating Cell Motility through Cytoskeletal Rearrangement
Abstract
:1. Introduction
2. Results
2.1. High TRPV4 Expression in Melanoma
2.2. Functional TRPV4 Is Required for A375 Metastasis
2.3. A Potent TRPV4 Inhibitor from the Natural Product Library: Baicalin
2.4. In Vitro and In Vivo Effects of Baicalin on Melanoma Metastasis
2.5. TRPV4 Promotes Melanoma Metastasis by Regulating Cell Motility
2.6. TRPV4 Regulates Melanoma Metastasis by Inducing Morphological Changes in Cells through the Src-Cofilin Axis
3. Discussion
4. Materials and Methods
4.1. Cell Lines and Cell Culture
4.2. Animals
4.3. Experimental Metastasis Model and Treatment Studies
4.4. Ca2+ Imaging
4.5. Cellular Thermal Shift Assay (CETSA)
4.6. Drug Affinity Responsive Target Stability Assay (DARTS)
4.7. Immunofluorescence
4.8. Wound-Healing Assay
4.9. Trans-Well Assay
4.10. Immunohistochemical Staining and Histopathological Analysis
4.11. Inverted Invasion Assay
4.12. SDS-PAGE and Western Blot Analysis
4.13. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Huang, S.; Yu, S.; Deng, R.; Liu, H.; Ding, Y.; Sun, Y.; Chen, W.; Wang, A.; Wei, Z.; Lu, Y. TRPV4 Promotes Metastasis in Melanoma by Regulating Cell Motility through Cytoskeletal Rearrangement. Int. J. Mol. Sci. 2022, 23, 15155. https://doi.org/10.3390/ijms232315155
Huang S, Yu S, Deng R, Liu H, Ding Y, Sun Y, Chen W, Wang A, Wei Z, Lu Y. TRPV4 Promotes Metastasis in Melanoma by Regulating Cell Motility through Cytoskeletal Rearrangement. International Journal of Molecular Sciences. 2022; 23(23):15155. https://doi.org/10.3390/ijms232315155
Chicago/Turabian StyleHuang, Shuai, Suyun Yu, Rui Deng, Huan Liu, Yushi Ding, Yifan Sun, Wenxing Chen, Aiyun Wang, Zhonghong Wei, and Yin Lu. 2022. "TRPV4 Promotes Metastasis in Melanoma by Regulating Cell Motility through Cytoskeletal Rearrangement" International Journal of Molecular Sciences 23, no. 23: 15155. https://doi.org/10.3390/ijms232315155