The Effect of the Stress-Signalling Mediator Triacontanol on Biochemical and Physiological Modifications in Dracocephalum forrestii Culture
Abstract
:1. Introduction
2. Results and Discussion
2.1. Effects of TRIA on Culture Growth
2.2. Determination of Chlorophyll Content
2.3. Genetic Stability
2.4. Effects of Triacontanol on Activities of Antioxidant Enzymes
2.5. Effects of Triacontanol Phenolic Compounds Content
3. Material and Methods
3.1. Plant Material and Experimental Setup
3.2. Measurement of Growth and Chlorophyll Content
3.3. Genetic Stability
3.4. Determination of Phenolic Compound Content
3.5. Estimation of Activities of Antioxidant Enzymes
3.6. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Activity of Antioxidant Enzymes (U/mg of Protein) | |||
---|---|---|---|
Medium with | POD | SOD | CAT |
TRIA 2.5 | 7.81 ± 0.64 d | 25.11 ± 0.15 b | 35.17 ± 0.15 d |
TRIA 5 | 15.81 ± 0.31 a | 29.62 ± 0.21 a | 84.98 ± 0.27 a |
TRIA 10 | 7.39 ± 0.61 d | 23.48 ± 0.09 c | 71.72 ± 0.28 c |
C | 3.21 ± 0.02 f | 12.10 ± 0.08 g | 11.75 ± 0.09 g |
TRIA 2.5-BI | 9.54 ± 0.70 c | 20.05 ± 0.11 d | 37.15 ± 0.19 e |
TRIA 5-BI | 11.14 ± 0.11 b | 16.23 ± 0.11 e | 78.07 ± 0.49 b |
TRIA 10-BI | 5.07 ± 0.40 e | 15.78 ± 0.18 f | 78.49 ± 0.23 b |
C-BI | 5.90 ± 0.09 e | 15.03 ± 0.12 f | 22.48 ± 0.18 f |
Compound | TRIA 2.5 | TRIA 5 | TRIA 10 | C | TRIA 2.5-BI | TRIA 5-BI | TRIA 10-BI | C-BI |
---|---|---|---|---|---|---|---|---|
chlorogenic acid | 0.53 ± 0.01 c | 0.31 ± 0.01 d | 0.11 ± 0.03 f | 1.64 ± 0.05 a | 0.15 ± 0.003 e | 0.18 ± 0.001 e | 0.19 ± 0.003 e | 0.60 ± 0.013 b |
caffeic acid | 0.03 ± 0.002 c | 0.03 ± 0.001 c | 0.04 ± 0.01 bc | 0.04 ± 0.002 bc | 0.10 ± 0.002 a | 0.09 ± 0.001 a | 0.09 ± 0.002 a | 0.05 ± 0.001 b |
salvianolic acid I/H and salvianolic acid E | 0.45 ± 0.01 b | 0.28 ± 0.01 d | 0.28 ± 0.01 d | 0.67 ± 0.02 a | 0.36 ± 0.003 c | 0.41 ± 0.01 b | 0.44 ± 0.01 b | 0.45 ± 0.04 b |
rosmarinic acid | 9.51 ± 0.22 d | 9.19 ± 0.23 d | 11.30 ± 0.02 b | 5.02 ± 0.01 f | 10.67 ± 0.21 c | 10.67 ± 0.19 c | 14.79 ± 0.31 a | 6.47 ± 0.01 e |
salvianolic acid B | 0.19 ± 0.001 e | 0.19 ± 0.01 e | 0.28 ± 0.002 c | 0.23 ± 0.01 d | 0.47 ± 0.01 a | 0.27 ± 0.001 c | 0.21 ± 0.00 d | 0.37 ± 0.01 b |
methyl rosmarinate | 0.28 ± 0.01 f | 0.39 ± 0.003 e | 0.48 ± 0.03 d | 0.54 ± 0.003 cd | 0.97 ± 0.04 a | 0.72 ± 0.03 b | 0.72 ± 0.01 b | 0.59 ± 0.1 c |
Total phenol content | 10.96 ± 0.1 c | 10.39 ± 0.3 c | 12.52 ± 0.2 b | 8.14 ± 0.2 d | 12.72 ± 0.1 b | 12.35 ± 0.2 b | 19.44 ± 0.34 a | 8.53 ± 0.1 d |
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Weremczuk-Jeżyna, I.; Hnatuszko-Konka, K.; Lebelt, L.; Piotrowska, D.G.; Grzegorczyk-Karolak, I. The Effect of the Stress-Signalling Mediator Triacontanol on Biochemical and Physiological Modifications in Dracocephalum forrestii Culture. Int. J. Mol. Sci. 2022, 23, 15147. https://doi.org/10.3390/ijms232315147
Weremczuk-Jeżyna I, Hnatuszko-Konka K, Lebelt L, Piotrowska DG, Grzegorczyk-Karolak I. The Effect of the Stress-Signalling Mediator Triacontanol on Biochemical and Physiological Modifications in Dracocephalum forrestii Culture. International Journal of Molecular Sciences. 2022; 23(23):15147. https://doi.org/10.3390/ijms232315147
Chicago/Turabian StyleWeremczuk-Jeżyna, Izabela, Katarzyna Hnatuszko-Konka, Liwia Lebelt, Dorota G. Piotrowska, and Izabela Grzegorczyk-Karolak. 2022. "The Effect of the Stress-Signalling Mediator Triacontanol on Biochemical and Physiological Modifications in Dracocephalum forrestii Culture" International Journal of Molecular Sciences 23, no. 23: 15147. https://doi.org/10.3390/ijms232315147