Functional Analyses of a Small Secreted Cysteine-Rich Protein ThSCSP_14 in Tilletia horrida
Abstract
:1. Introduction
2. Results
2.1. ThSCSP_14 in T. horrida Induces Cell Death in N. benthamiana
2.2. ThSCSP_14 Is Unique to Tilletia fungi
2.3. Functional Validation of the Predicted SP of ThSCSP_14
2.4. ThSCSP_14 Expression during T. horrida Infection
2.5. ThSCSP_14-triggered Cell Death in N. benthamiana Depends on SGT1, RAR1, HSP90, and SERK3/Bak1
2.6. Subcellular Localization of ThSCSP_14
2.7. ThSCSP_14 Triggers Plant Immunity Responses
3. Discussion
4. Materials and Methods
4.1. Strains, Plant Materials, and Growth Conditions
4.2. Candidate Effectors
4.3. Plasmid Construction of Candidate Effectors
4.4. Transient Expression Assays
4.5. Secretory Function Assays of ThSCSP_14′s SP
4.6. RNA Extraction and Gene Expression Analysis
4.7. Western Blot Analysis
4.8. Trypan Blue Staining, H2O2 Activity, and Callose Deposition Observation
4.9. Virus-Induced Gene Silencing in N. benthamiana
4.10. Subcellular Localization
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Shu, X.; Xu, D.; Jiang, Y.; Liang, J.; Xiang, T.; Wang, Y.; Zhang, W.; Han, X.; Jiao, C.; Zheng, A.; et al. Functional Analyses of a Small Secreted Cysteine-Rich Protein ThSCSP_14 in Tilletia horrida. Int. J. Mol. Sci. 2022, 23, 15042. https://doi.org/10.3390/ijms232315042
Shu X, Xu D, Jiang Y, Liang J, Xiang T, Wang Y, Zhang W, Han X, Jiao C, Zheng A, et al. Functional Analyses of a Small Secreted Cysteine-Rich Protein ThSCSP_14 in Tilletia horrida. International Journal of Molecular Sciences. 2022; 23(23):15042. https://doi.org/10.3390/ijms232315042
Chicago/Turabian StyleShu, Xinyue, Deze Xu, Yuqi Jiang, Juan Liang, Ting Xiang, Yuxuan Wang, Weike Zhang, Xue Han, Chunhai Jiao, Aiping Zheng, and et al. 2022. "Functional Analyses of a Small Secreted Cysteine-Rich Protein ThSCSP_14 in Tilletia horrida" International Journal of Molecular Sciences 23, no. 23: 15042. https://doi.org/10.3390/ijms232315042