Gender Dimorphism in Hepatic Carcinogenesis-Related Gene Expression Associated with Obesity as a Low-Grade Chronic Inflammatory Disease
Abstract
:1. Introduction
2. Results
2.1. Sex-Biased Carcinogenesis-Related Gene Expression in Livers from Obese Rats
2.2. Sexual Dimorphism in SURVIVIN/BIRC5 Oncogene Expression According to the Degree of Adiposity in Animals and Humans
3. Discussion
4. Methods and Materials
4.1. Animals with Diet-Induced Obesity (DIO)
4.2. Patients with Obesity and Normal Weight Individuals
4.3. Gene Expression Assessment
4.4. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lean | Obese | |||
---|---|---|---|---|
Sex | Male | Female | Male | Female |
n | 10 | 10 | 10 | 10 |
Age (weeks) | 8 | 6 | 8 | 6 |
Body weight (g) | 562.3 ± 15.2 | 226.4 ± 10.8 # | 663.5 ± 76.4 * | 245.8 ± 10.3 *,# |
Fat mass (g) | 52.4 ± 15.6 | 16.0 ± 4.26 # | 146.8 ± 59.7 * | 30.4 ± 5.32 *,# |
Fat mass (%) | 9.30 ± 2.67 | 7.02 ± 1.71 # | 21.6 ± 6.50 * | 12.4 ± 2.12 *,# |
Fat-free mass (g) | 420.0 ± 16.1 | 173.3 ± 10.1 # | 424.7 ± 27.6 | 170.6 ± 8.64 # |
Fat-free mass (%) | 74.7 ± 2.34 | 76.6 ± 4.08 | 64.5 ± 6.23 * | 69.4 ± 2.73 *,# |
Lean | Obese | ANOVA p-Value | |||||
---|---|---|---|---|---|---|---|
GENE | Male | Female | Male | Female | Group | Sex | Group * Sex |
SURVIVIN/BIRC5 | 1.06 ± 0.39 | 1.02 ± 0.19 | 2.04 ± 0.89 | 1.37 ± 0.37 | <0.001 * | 0.041 * | 0.068 |
MYC | 1.02 ± 0.20 | 1.01 ± 0.15 | 1.40 ± 0.24 | 1.16 ± 0.33 | 0.003 * | 0.144 | 0.183 |
GSTM2 | 1.01 ± 0.16 | 1.02 ± 0.18 | 0.70 ± 0.34 | 0.71 ± 0.11 | <0.001 * | 0.931 | 0.981 |
SIRT1 | 1.06 ± 0.46 | 1.07 ± 0.44 | 0.51 ± 0.24 | 0.59 ± 0.09 | <0.001 * | 0.807 | 0.899 |
SIRT6 | 1.08 ± 0.46 | 1.08 ± 0.45 | 0.67 ± 0.25 | 0.55 ± 0.12 | <0.001 * | 0.604 | 0.604 |
TGFB1 | 1.05 ± 0.30 | 1.06 ± 0.38 | 0.60 ± 0.17 | 0.72 ± 0.12 | <0.001 * | 0.424 | 0.503 |
TP53 | 1.11 ± 0.50 | 1.04 ± 0.30 | 0.62 ± 0.23 | 0.91 ± 0.16 | 0.004 * | 0.323 | 0.085 |
PTEN | 1.05 ± 0.33 | 1.02 ± 0.22 | 0.74 ± 0.30 | 0.75 ± 0.14 | 0.001 * | 0.911 | 0.797 |
Normal Weight | Obesity | |||
---|---|---|---|---|
Men | Women | Men | Women | |
n | 10 | 19 | 10 | 12 |
Age (years) | 40.2 ± 8.82 | 33.5 ± 9.00 | 42.50 ± 10.8 | 35.7 ± 10.8 |
Height (m) | 1.73 ± 0.06 | 1.65 ± 0.07 | 1.74 ± 0.05 | 1.64 ± 0.06 |
Body weight (kg) | 67.2 ± 7.81 | 57.6 ± 4.07 | 114.2 ± 20.2 * | 113.2 ± 21.6 * |
BMI (kg/m2) | 23.5 ± 2.33 | 21.4 ± 1.72 | 38.0 ± 6.90 * | 41.9 ± 7.70 * |
WC (cm) | 86.2 ± 7.66 | 76.9 ± 7.89 | 120.8 ± 19.6 * | 122.8 ± 15.5 * |
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Izquierdo, A.G.; Carreira, M.C.; Rodriguez-Carnero, G.; Perez-Lois, R.; Seoane, L.M.; Casanueva, F.F.; Crujeiras, A.B. Gender Dimorphism in Hepatic Carcinogenesis-Related Gene Expression Associated with Obesity as a Low-Grade Chronic Inflammatory Disease. Int. J. Mol. Sci. 2022, 23, 15002. https://doi.org/10.3390/ijms232315002
Izquierdo AG, Carreira MC, Rodriguez-Carnero G, Perez-Lois R, Seoane LM, Casanueva FF, Crujeiras AB. Gender Dimorphism in Hepatic Carcinogenesis-Related Gene Expression Associated with Obesity as a Low-Grade Chronic Inflammatory Disease. International Journal of Molecular Sciences. 2022; 23(23):15002. https://doi.org/10.3390/ijms232315002
Chicago/Turabian StyleIzquierdo, Andrea G., Marcos C. Carreira, Gemma Rodriguez-Carnero, Raquel Perez-Lois, Luisa M. Seoane, Felipe F. Casanueva, and Ana B. Crujeiras. 2022. "Gender Dimorphism in Hepatic Carcinogenesis-Related Gene Expression Associated with Obesity as a Low-Grade Chronic Inflammatory Disease" International Journal of Molecular Sciences 23, no. 23: 15002. https://doi.org/10.3390/ijms232315002