Identification and Functional Analysis of bZIP Genes in Cotton Response to Drought Stress
Abstract
:1. Introduction
2. Results
2.1. Identification of bZIP Transcription Factors in G. Hirsutum
2.2. Phylogenetic Analysis, Prediction of Conserved Motifs, and Display of Gene Structure
2.3. DNA-Binding Site Specificity Prediciton of GhbZIP Transcription Factors
2.4. Dimerization Properties Prediction of bZIP Transcription Factors
2.5. Expression Analysis of GhbZIP Family Members in Cotton under Drought Treatments
2.6. GhbZIP177 Conferred Drought Resistance in Cotton
3. Discussion
4. Materials and Method
4.1. Identification of bZIP Family Genes in Gossypium spp
4.2. Chromosomal Locations and Gene Duplication Analysis
4.3. Phylogeny Analysis bZIP
4.4. Sequence Analysis
4.5. Expression Profiles
4.6. Functional Analysis of GhbZIP177 in Cotton Response to Drought Stress
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Group | No. of Members | Characteristic Features of bZIP Domain | Putative Binding Site |
---|---|---|---|
A | 41 | *********MIK************QAY*** | ABREs with the core ACGT or others containing GCGT/AAGT |
B | 1 | **********RNRESAQLSR********** | G- and C-boxes with equal affinity |
C | 13 | ***********************QAHLEE* | Hybrid ACGT elements such as G/C, G/A, and C/G boxes |
D | 32 | ********LAQN**AA*KSR***KAYVQQ* | C-box sequence |
E | 8 | *********A**************QYIS(/A)E* | Unknown |
F/K | 10 | **************A**************** | C-box elements |
G/M/S | 62 | ***********N**SA**SR********** | G-box and/or G-box-like sequences |
H | 2 | ***********NRVSAQQAR********** | G-box-like sequences |
I | 25 | *******************K********** | Sequences other than those containing a palindromic ACGT core, such as CCA/TGG repeats |
J | 2 | *******************I********** | Unknown |
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Zhang, B.; Feng, C.; Chen, L.; Li, B.; Zhang, X.; Yang, X. Identification and Functional Analysis of bZIP Genes in Cotton Response to Drought Stress. Int. J. Mol. Sci. 2022, 23, 14894. https://doi.org/10.3390/ijms232314894
Zhang B, Feng C, Chen L, Li B, Zhang X, Yang X. Identification and Functional Analysis of bZIP Genes in Cotton Response to Drought Stress. International Journal of Molecular Sciences. 2022; 23(23):14894. https://doi.org/10.3390/ijms232314894
Chicago/Turabian StyleZhang, Boyang, Cheng Feng, Lin Chen, Baoqi Li, Xianlong Zhang, and Xiyan Yang. 2022. "Identification and Functional Analysis of bZIP Genes in Cotton Response to Drought Stress" International Journal of Molecular Sciences 23, no. 23: 14894. https://doi.org/10.3390/ijms232314894