Effects of Pro-Inflammatory Cytokines on Hepatic Metabolism in Primary Human Hepatocytes
Abstract
:1. Introduction
2. Results
2.1. Inflammatory Cytokines on Ammonia Metabolism
2.2. Inflammatory Cytokines on CYP Activities
2.3. Inflammatory Cytokines on Phase II Enzyme (UGT1A1) Activity
2.4. Inflammatory Cytokines on Drug Transporter (BSEP) Activity
3. Discussion
4. Materials and Methods
4.1. Primary Hepatocyte Cultures
4.2. Cytotoxicity Evaluation
4.3. Apoptosis Evaluation
4.4. Ammonia Metabolism Assay
4.5. Cytochrome P450 Enzyme Activities
4.6. Phase II in Vitro Evaluation
4.7. Evaluation of BSEP Activity
4.8. Protein Amount Quantification
4.9. dsDNA Amount Quantification
4.10. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gramignoli, R.; Ranade, A.R.; Venkataramanan, R.; Strom, S.C. Effects of Pro-Inflammatory Cytokines on Hepatic Metabolism in Primary Human Hepatocytes. Int. J. Mol. Sci. 2022, 23, 14880. https://doi.org/10.3390/ijms232314880
Gramignoli R, Ranade AR, Venkataramanan R, Strom SC. Effects of Pro-Inflammatory Cytokines on Hepatic Metabolism in Primary Human Hepatocytes. International Journal of Molecular Sciences. 2022; 23(23):14880. https://doi.org/10.3390/ijms232314880
Chicago/Turabian StyleGramignoli, Roberto, Aarati R. Ranade, Raman Venkataramanan, and Stephen C. Strom. 2022. "Effects of Pro-Inflammatory Cytokines on Hepatic Metabolism in Primary Human Hepatocytes" International Journal of Molecular Sciences 23, no. 23: 14880. https://doi.org/10.3390/ijms232314880