Fabrication and In Vitro Characterization of Novel Hydroxyapatite Scaffolds 3D Printed Using Polyvinyl Alcohol as a Thermoplastic Binder
Abstract
:1. Introduction
2. Results
2.1. MTT Assay
2.2. LDH Release Assay
2.3. Characteristics of Used Material
2.4. Surface Morphology of Scaffolds Sintered at Elevated Temperatures
2.5. Resulting Scaffolds after Biocolonization with MSC
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Methods
4.2.1. Direct Contact Cytotoxicity Assay
4.2.2. MTT Assay
4.2.3. LDH Release Assay
4.2.4. Methods of Sample Preparation for Scanning Electron Microscopy
4.2.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Thurzo, A.; Gálfiová, P.; Nováková, Z.V.; Polák, Š.; Varga, I.; Strunga, M.; Urban, R.; Surovková, J.; Leško, Ľ.; Hajdúchová, Z.; et al. Fabrication and In Vitro Characterization of Novel Hydroxyapatite Scaffolds 3D Printed Using Polyvinyl Alcohol as a Thermoplastic Binder. Int. J. Mol. Sci. 2022, 23, 14870. https://doi.org/10.3390/ijms232314870
Thurzo A, Gálfiová P, Nováková ZV, Polák Š, Varga I, Strunga M, Urban R, Surovková J, Leško Ľ, Hajdúchová Z, et al. Fabrication and In Vitro Characterization of Novel Hydroxyapatite Scaffolds 3D Printed Using Polyvinyl Alcohol as a Thermoplastic Binder. International Journal of Molecular Sciences. 2022; 23(23):14870. https://doi.org/10.3390/ijms232314870
Chicago/Turabian StyleThurzo, Andrej, Paulína Gálfiová, Zuzana Varchulová Nováková, Štefan Polák, Ivan Varga, Martin Strunga, Renáta Urban, Jana Surovková, Ľuboš Leško, Zora Hajdúchová, and et al. 2022. "Fabrication and In Vitro Characterization of Novel Hydroxyapatite Scaffolds 3D Printed Using Polyvinyl Alcohol as a Thermoplastic Binder" International Journal of Molecular Sciences 23, no. 23: 14870. https://doi.org/10.3390/ijms232314870