Sex Steroid Hormone Analysis in Human Tear Fluid Using a Liquid Chromatography—Mass Spectrometry Method
Abstract
:1. Introduction
2. Results
3. Discussion
4. Methods and Materials
4.1. Tear Fluid Sample Collection and Preparation
4.2. LC–MS/MS Analysis
4.3. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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E2 (pmol/L) | E1 (pmol/L) | P4 (nmol/L) | T4 (nmol/L) | A4 (nmol/L) | DHEA (nmol/L) | |
---|---|---|---|---|---|---|
H2O blank | <5 | <5 | <0.05 | <0.01 | <0.01 | <0.1 |
PBS blank | <5 | <5 | <0.05 | <0.01 | <0.01 | <0.1 |
Tear fluid | <5 | 11 | 0.9 | <0.01 | 0.5 | 0.6 |
E2 (pmol/L) |
E1 (pmol/L) |
P4 (nmol/L) |
T4 (nmol/L) |
A4 (nmol/L) |
DHEA (nmol/L) | ||
---|---|---|---|---|---|---|---|
blank | <5 | <5 | <0.01 | <0.01 | <0.01 | <0.1 | |
female | TF 1 n = 5 | <5 | 24 (0–173.4) | 0.04 (0–8.5) | <0.01 | 0.68 (0.4–1.5) | 0.8 (0–2.8) |
TF 2 n = 4 | <5 | 48 (44–200.1) | 1.12 (0–3.94) | <0.01 | 1.26 (0.8–2.0) | 0.8 (0–1.2) | |
Serum 1 n = 5 | 131 (15–698) | 162 (52–294) | 0.18 (0.1–1.1) | 1.05 (0.7–1.4) | 4.75 (3.5–5.6) | 16.9 (14.8–26.4) | |
Serum 2 n = 4 | 324 (248–434) | 282 (201–368) | 21.75 (0.4–38.9) | 1.28 (1–2.1) | 4.67 (3.4–11.6) | 21.1 (14.6–30.1) | |
male | TF n = 5 | <5 | 33.35 | <0.01 | 0.2 (0.1–0.4) | 1.6 (1.1–2.3) | 2.01 |
Serum n = 5 | 58 (21–80) | 67 (59–137) | 0.18 (0.1–0.4) | 18 (10–25.3) | 3.37 (1.3–4.1) | 20 (6.1–27.4) |
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Robciuc, A.; Savolainen-Peltonen, H.; Haanpää, M.; Moilanen, J.A.O.; Mikkola, T.S. Sex Steroid Hormone Analysis in Human Tear Fluid Using a Liquid Chromatography—Mass Spectrometry Method. Int. J. Mol. Sci. 2022, 23, 14864. https://doi.org/10.3390/ijms232314864
Robciuc A, Savolainen-Peltonen H, Haanpää M, Moilanen JAO, Mikkola TS. Sex Steroid Hormone Analysis in Human Tear Fluid Using a Liquid Chromatography—Mass Spectrometry Method. International Journal of Molecular Sciences. 2022; 23(23):14864. https://doi.org/10.3390/ijms232314864
Chicago/Turabian StyleRobciuc, Alexandra, Hanna Savolainen-Peltonen, Mikko Haanpää, Jukka A. O. Moilanen, and Tomi S. Mikkola. 2022. "Sex Steroid Hormone Analysis in Human Tear Fluid Using a Liquid Chromatography—Mass Spectrometry Method" International Journal of Molecular Sciences 23, no. 23: 14864. https://doi.org/10.3390/ijms232314864