Chemical Markers of Human Tendon Health Identified Using Raman Spectroscopy: Potential for In Vivo Assessment
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Tendon Sample Collection
4.2. Raman Spectroscopy Instrumentation
4.3. Raman Data Treatment and Analysis
4.4. Biochemical Analysis
4.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Raman Band | Pentosidine | Glucosepane | ||
---|---|---|---|---|
Achilles | Tibialis | Achilles | Tibialis | |
850 cm−1 | 0.01 | −0.01 | −0.09 | −0.50 |
877 cm−1 | 0.75 | 0.82 | 0.66 | 0.79 |
918 cm−1 | 0.73 | 0.73 | 0.69 | 0.91 |
931 cm−1 | 0.34 | 0.72 | 0.17 | 0.81 |
951 cm−1 | 0.75 | 0.85 | 0.69 | 0.88 |
1002 cm−1 | −0.68 | −0.09 | −0.71 | −0.54 |
1130 cm−1 | 0.75 | 0.72 | 0.74 | 0.93 |
1270 cm−1 | 0.74 | 0.72 | 0.53 | 0.74 |
1293 cm−1 | 0.78 | 0.88 | 0.81 | 0.86 |
1324 cm−1 | 0.74 | 0.75 | 0.77 | 0.92 |
1450 cm−1 | −0.83 | 0.04 | 0.77 | −0.66 |
1660 cm−1 | −0.80 | 0.21 | −0.76 | −0.55 |
Raman Band | Achilles Tendon | Tibialis Tendon | p-Value |
---|---|---|---|
825 cm−1 | 0.23 ± 0.05 | 0.38 ± 0.12 | <0.001 |
850 cm−1 | 0.56 ± 0.03 | 0.47 ± 0.07 | <0.001 |
877 cm−1 | 0.44 ± 0.05 | 0.51 ± 0.08 | <0.001 |
931 cm−1 | 0.66 ± 0.04 | 0.59 ± 0.06 | <0.001 |
1002 cm−1 | 0.66 ± 0.06 | 0.75 ± 0.11 | <0.001 |
1030 cm−1 | 0.69 ± 0.03 | 0.77 ± 0.07 | <0.001 |
1049 cm−1 | 0.52 ± 0.03 | 0.62 ± 0.05 | <0.001 |
1082 cm−1 | 0.50 ± 0.05 | 0.57 ± 0.05 | <0.001 |
1240 cm−1 | 0.97 ± 0.02 | 0.92 ± 0.03 | <0.001 |
1270 cm−1 | 0.93 ± 0.05 | 0.98 ± 0.05 | <0.001 |
1450 cm−1 | 1.11 ± 0.06 | 1.00 ± 0.07 | <0.001 |
1660 cm−1 | 0.69 ± 0.03 | 0.63 ± 0.04 | <0.001 |
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Yin, N.-H.; Parker, A.W.; Matousek, P.; Birch, H.L. Chemical Markers of Human Tendon Health Identified Using Raman Spectroscopy: Potential for In Vivo Assessment. Int. J. Mol. Sci. 2022, 23, 14854. https://doi.org/10.3390/ijms232314854
Yin N-H, Parker AW, Matousek P, Birch HL. Chemical Markers of Human Tendon Health Identified Using Raman Spectroscopy: Potential for In Vivo Assessment. International Journal of Molecular Sciences. 2022; 23(23):14854. https://doi.org/10.3390/ijms232314854
Chicago/Turabian StyleYin, Nai-Hao, Anthony W. Parker, Pavel Matousek, and Helen L. Birch. 2022. "Chemical Markers of Human Tendon Health Identified Using Raman Spectroscopy: Potential for In Vivo Assessment" International Journal of Molecular Sciences 23, no. 23: 14854. https://doi.org/10.3390/ijms232314854