ROS-Induced DNA-Damage and Autophagy in Oral Squamous Cell Carcinoma by Usnea barbata Oil Extract—An In Vitro Study
Abstract
:1. Introduction
2. Results
2.1. Atomic Force Microscopy
2.2. Rheology
2.3. Annexin V-FITC Apoptosis Assay
2.4. Total ROS Activity Assay
2.5. Enzymatic Activity of Caspase 3/7
2.6. Cell Cycle Assay
2.7. Nuclear Shrinkage and Autophagy
2.8. DNA Synthesis Assay
2.9. Principal Component Analysis
2.10. Antibacterial and Antifungal Activities
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Lichen Extract in Canola Oil
4.3. Physico-Chemical Properties of Usnea barbata (L.) F.H. Wigg Extract in Canola Oil
4.3.1. Atomic Force Microscopy
4.3.2. Rheology
4.4. In Vitro Cytotoxicity
4.4.1. Human Blood Cell Cultures
4.4.2. CLS-354 Cell Line, Cell Culture
4.4.3. Samples and Controls
4.4.4. Equipment
4.4.5. Data Analysis
4.5. Antibacterial and Antifungal Activities
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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CTR (mg/mL) | TRF (mg/mL) | P407 (mg/mL) | UBO/CNO (mg/mL) | ||||
---|---|---|---|---|---|---|---|
30.230 ± 0.630 | 122.330 ± 0.850 | 10.050 ± 0.180 | 50.133 ± 1.305 | 303.300 ± 15.275 | |||
1.511 ± 0.043 | 6.117 ± 0.042 | 0.500 ± 0.009 | 2.506 ± 0.065 | 15.167 ± 0.764 | |||
0.755 ± 0.022 | 4.893 ± 0.034 | 0.250 ± 0.004 | 1.253 ± 0.032 | 7.583 ± 0.382 | |||
0.377 ± 0.011 | 3.914 ± 0.027 | 0.125 ± 0.002 | 0.626 ± 0.016 | 3.790 ± 0.193 | |||
0.188 ± 0.005 | 3.131 ± 0.021 | 0.061 ± 0.001 | 0.315 ± 0.008 | 1.895 ± 0.097 | |||
0.094 ± 0.002 | 2.505 ± 0.017 | 0.031 ± 0.001 | 0.157 ± 0.004 | 0.947 ± 0.048 | |||
0.047 ± 0.002 | 2.004 ± 0.014 | 0.015 ± 0.001 | 0.078 ± 0.002 | 0.473 ± 0.024 | |||
0.023 ± 0.001 | 1.603 ± 0.011 | 0.007 ± 0.001 | 0.039 ± 0.001 | 0.237 ± 0.012 | |||
S. aureus | P. aeruginosa | ||||||
UBO | CNO | P407 | CTR | UBO | CNO | P407 | CTR |
* | |||||||
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Popovici, V.; Musuc, A.M.; Matei, E.; Karampelas, O.; Ozon, E.A.; Cozaru, G.C.; Schröder, V.; Bucur, L.; Aricov, L.; Anastasescu, M.; et al. ROS-Induced DNA-Damage and Autophagy in Oral Squamous Cell Carcinoma by Usnea barbata Oil Extract—An In Vitro Study. Int. J. Mol. Sci. 2022, 23, 14836. https://doi.org/10.3390/ijms232314836
Popovici V, Musuc AM, Matei E, Karampelas O, Ozon EA, Cozaru GC, Schröder V, Bucur L, Aricov L, Anastasescu M, et al. ROS-Induced DNA-Damage and Autophagy in Oral Squamous Cell Carcinoma by Usnea barbata Oil Extract—An In Vitro Study. International Journal of Molecular Sciences. 2022; 23(23):14836. https://doi.org/10.3390/ijms232314836
Chicago/Turabian StylePopovici, Violeta, Adina Magdalena Musuc, Elena Matei, Oana Karampelas, Emma Adriana Ozon, Georgeta Camelia Cozaru, Verginica Schröder, Laura Bucur, Ludmila Aricov, Mihai Anastasescu, and et al. 2022. "ROS-Induced DNA-Damage and Autophagy in Oral Squamous Cell Carcinoma by Usnea barbata Oil Extract—An In Vitro Study" International Journal of Molecular Sciences 23, no. 23: 14836. https://doi.org/10.3390/ijms232314836