Dominant Elongase Activity of Elovl5a but Higher Expression of Elovl5b in Common Carp (Cyprinus carpio)
Abstract
:1. Introduction
2. Results
2.1. The Structure Difference between Common Carp Elovl5a and Elovl5b
2.2. Dominant Elongase Activities of Elovl5a Compared with Elovle5b
2.3. In Silico Molecular Docking Difference between Elovl5a and Elovl5b
2.4. Higher Expression Levels of Elovl5b Than Elovl5a
2.5. Correlation between the Larvae PUFA Contents and the Expressions of Two Genes
2.6. Promoter Activity Analysis of Elovl5a and Elovl5b
3. Discussion
4. Materials and Methods
4.1. Animals and Tissue Collection
4.2. Cloning of Elovl5a and Elovl5b cDNAs
4.3. Sequence and Phylogenetic Analysis
4.4. Functional Characterization of the Common Carp Elovl5a and Elovl5b in Yeast
4.5. Structure Modelling and Docking of Elovl5a and Elovl5b
4.6. Spatial-Temporal Expression of Elovl5a and Elovl5b
4.7. Fatty Acid Analysis of Common Carp Embryos at Different Developmental Stages
4.8. Identifying the Core Promoters of Common Carp Elovl5a and Elovl5b
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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FA Substrate | Product | Elovl5a Conversion (%) | Elovl5b Conversion (%) | Activity |
---|---|---|---|---|
C18:2n-6 | C20:2n-6 | 11.63 | 8.42 | C18→C20 |
C18:3n-6 | C20:3n-6 | 74.24 | 69.63 | C18→C20 |
C24:3n-6 | 8.45 | 1.14 | C22→C24 | |
Total | 82.69 | 70.74 | ||
C18:4n-3 | C20:4n-3 | 44.91 | 43.91 | C18→C20 |
C18:3n-3 | C20:3n-3 | 36 | 22.97 | C18→C20 |
C24:3n-3 | 9.2 | 3.9 | C22→C24 | |
Total | 45.2 | 26.87 | ||
C20:5n-3 | C22:5n-3 | 25.12 | 26.52 | C20→C22 |
C24:5n-3 | 4.83 | 3.68 | C22→C24 | |
Total | 29.95 | 30.2 | ||
C20:4n-6 | C22:4n-6 | 17.90 | 13.29 | C20→C22 |
C24:4n-6 | 5.02 | 2.92 | C22→C24 | |
Total | 22.92 | 16.21 | ||
C22:4n-6 | C24:4n-6 | 4.66 | 3.87 | C22→C24 |
C22:5n-3 | C24:5n-3 | 8.24 | 6.00 | C22→C24 |
FA Substrates | 0 hpf | 24 hpf | 48 hpf | 72 hpf | 96 hpf | 120 hpf |
---|---|---|---|---|---|---|
C18:2n-6 | 121.22 | 108.9 | 104.32 | 85.53 | 68.28 | 37.92 |
C20:2n-6 | 13.26 | 11.67 | 11.9 | 10.99 | 10.39 | 7.91 |
C20:3n-6 | 75.96 | 19.89 | 19.49 | 17.58 | 16.17 | 11.6 |
C22:4n-6 | 12.3 | 11.26 | 10.87 | 10.18 | 10.42 | 8.12 |
elovl5-related n-6 PUFAs | 222.74 | 151.72 | 146.58 | 124.28 | 105.26 | 65.55 |
total n-6 PUFA products | 101.52 | 42.82 | 42.26 | 38.75 | 36.98 | 27.63 |
n-6 products/substrate ratio | 0.84 | 0.39 | 0.405 | 0.453 | 0.54 | 0.73 |
C18:3n-3 | 5.71 | 5.06 | 4.56 | 3.06 | 2.08 | 1.06 |
C20:3n-3 | 0.94 | 1.08 | 1.02 | 0.85 | 0.83 | 0.71 |
C20:4n-3 | 0.59 | 0.47 | 0.48 | 0.4 | 0.37 | 0.17 |
C22:5n-3 | 1.47 | 3.7 | 3.54 | 3.86 | 3.65 | 3.04 |
elovl5-related n-3 PUFAs | 8.71 | 10.31 | 9.6 | 8.17 | 6.93 | 4.98 |
total n-3 PUFA products | 3 | 5.25 | 5.04 | 5.11 | 4.85 | 3.92 |
n-3 products/substrate ratio | 0.525 | 1.04 | 1.11 | 1.67 | 2.33 | 3.70 |
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Zhao, R.; Wang, Y.-X.; Yang, C.-R.; Li, S.-Q.; Li, J.-C.; Sun, X.-Q.; Wang, H.-W.; Wang, Q.; Zhang, Y.; Li, J.-T. Dominant Elongase Activity of Elovl5a but Higher Expression of Elovl5b in Common Carp (Cyprinus carpio). Int. J. Mol. Sci. 2022, 23, 14666. https://doi.org/10.3390/ijms232314666
Zhao R, Wang Y-X, Yang C-R, Li S-Q, Li J-C, Sun X-Q, Wang H-W, Wang Q, Zhang Y, Li J-T. Dominant Elongase Activity of Elovl5a but Higher Expression of Elovl5b in Common Carp (Cyprinus carpio). International Journal of Molecular Sciences. 2022; 23(23):14666. https://doi.org/10.3390/ijms232314666
Chicago/Turabian StyleZhao, Ran, Ya-Xin Wang, Chen-Ru Yang, Shang-Qi Li, Jin-Cheng Li, Xiao-Qing Sun, Hong-Wei Wang, Qi Wang, Yan Zhang, and Jiong-Tang Li. 2022. "Dominant Elongase Activity of Elovl5a but Higher Expression of Elovl5b in Common Carp (Cyprinus carpio)" International Journal of Molecular Sciences 23, no. 23: 14666. https://doi.org/10.3390/ijms232314666