In Vitro versus Cryo-Induced Capacitation of Bovine Spermatozoa, Part 2: Changes in the Expression Patterns of Selected Transmembrane Channels and Protein Kinase A
Abstract
:1. Introduction
2. Results
2.1. Sperm Motility
2.2. Capacitation Status
2.3. Gene Expression Patterns
2.4. Protein Expression Patterns
3. Discussion
4. Materials and Methods
4.1. Biological Material and Culture
4.2. Cryopreservation Procedure
4.3. Thawing and Washing Procedure
4.4. Computer-Assisted Sperm Analysis
4.5. Capacitation Patterns
4.6. RNA Isolation and cDNA Synthesis
4.7. Real-Time qPCR
4.8. Western Blot
- CATSPER1 Polyclonal Antibody (#PA5-75788; Invitrogen, Waltham, MA, USA), source: rabbit, dilution 1:1000 in 5% milk/TBS/0.1% Tween-20;
- CATSPER2 Polyclonal Antibody (#PA5-41072; Invitrogen, Waltham, MA, USA), source: rabbit, dilution 1:1000 in 5% milk/TBS/0.1% Tween-20;
- PKA alpha Antibody (#PA5-17626; Invitrogen, Waltham, MA, USA), source: rabbit, dilution 1:1000 in 5% milk/TBS/0.1% Tween-20;
- Anti-Na+/HCO3− Contransporter Polyclonal Antibody (#AB3212-I; EMD Milipore Corporation; Temecula, CA, USA), source: rabbit, dilution 1:500 in 5% milk/TBS/0.1% Tween-20.
4.9. Data Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primer Name | Primer Sequences | Accession Number | Amplicon Size |
---|---|---|---|
Bov CATSPER1-F | TACTCTGACCCCAAACGCTT | XM_024987569.1 | 100 bp |
Bov CATSPER1-R | GGCTGTCCAGGTAGATGAGG | ||
Bov CATSPER2-F | CCTCAAGAGCATGACCTTCC | NM_001192477.1 | 103 bp |
Bov CATSPER2-R | GCGAGTTGAACGGGTGTAAT | ||
Bov GAPDH-F | GATGGTGAAGGTCGGAGTGAAC | NM_001034034.2 | 100 bp |
Bov GAPDH-R | GTCATTGATGGCGACGATGT | ||
Bov NBC-F | CAGCCATGACCCACAGGAAT | AF308160.1 | 146 bp |
Bov NBC-R | AGTCTACCTCGCCAACAAGC | ||
Bov PKA-F | GAAGCCCAAAGCCAGCTCTA | NM_174236.1 | 109 bp |
Bov PKA-R | TTTGATTGAGTCCCAGGCCC |
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Benko, F.; Fialková, V.; Žiarovská, J.; Ďuračka, M.; Lukáč, N.; Tvrdá, E. In Vitro versus Cryo-Induced Capacitation of Bovine Spermatozoa, Part 2: Changes in the Expression Patterns of Selected Transmembrane Channels and Protein Kinase A. Int. J. Mol. Sci. 2022, 23, 14646. https://doi.org/10.3390/ijms232314646
Benko F, Fialková V, Žiarovská J, Ďuračka M, Lukáč N, Tvrdá E. In Vitro versus Cryo-Induced Capacitation of Bovine Spermatozoa, Part 2: Changes in the Expression Patterns of Selected Transmembrane Channels and Protein Kinase A. International Journal of Molecular Sciences. 2022; 23(23):14646. https://doi.org/10.3390/ijms232314646
Chicago/Turabian StyleBenko, Filip, Veronika Fialková, Jana Žiarovská, Michal Ďuračka, Norbert Lukáč, and Eva Tvrdá. 2022. "In Vitro versus Cryo-Induced Capacitation of Bovine Spermatozoa, Part 2: Changes in the Expression Patterns of Selected Transmembrane Channels and Protein Kinase A" International Journal of Molecular Sciences 23, no. 23: 14646. https://doi.org/10.3390/ijms232314646