Mobilization of Hematopoietic Stem and Progenitor Cells during Dengue Virus Infection
Abstract
:1. Introduction
2. Results
2.1. Mobilization of HSPCs in Dengue-Infected Patients
2.2. Homing Markers Expression in HSPCs during Dengue Infection
2.3. HSPCs in Dengue Patients Were Permissive for DENV Infection
3. Discussion
4. Materials and Methods
4.1. Ethical Statement and Study Cohort
4.2. White Blood Cells Preparation
4.3. Fluorochrome Conjugated DENV NS1 Antibody
4.4. Flow Cytometric Analysis
4.5. Gating Strategy for HSPCs, Homing HSPCs, and DENV Infected or Uninfected HSPCs
4.6. HSPCs Isolation
4.7. Virus Titration
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Puc, I.; Ho, T.-C.; Chien, Y.-W.; Tan, S.-S.; Fong, Y.-C.; Chen, Y.-J.; Wang, S.-H.; Li, Y.-H.; Chen, C.-H.; Chen, P.-L.; et al. Mobilization of Hematopoietic Stem and Progenitor Cells during Dengue Virus Infection. Int. J. Mol. Sci. 2022, 23, 14330. https://doi.org/10.3390/ijms232214330
Puc I, Ho T-C, Chien Y-W, Tan S-S, Fong Y-C, Chen Y-J, Wang S-H, Li Y-H, Chen C-H, Chen P-L, et al. Mobilization of Hematopoietic Stem and Progenitor Cells during Dengue Virus Infection. International Journal of Molecular Sciences. 2022; 23(22):14330. https://doi.org/10.3390/ijms232214330
Chicago/Turabian StylePuc, Irwin, Tzu-Chuan Ho, Yu-Wen Chien, Sia-Seng Tan, Yu-Cin Fong, Yi-Ju Chen, Sheng-Hsuan Wang, Yun-Hsuan Li, Chun-Hong Chen, Po-Lin Chen, and et al. 2022. "Mobilization of Hematopoietic Stem and Progenitor Cells during Dengue Virus Infection" International Journal of Molecular Sciences 23, no. 22: 14330. https://doi.org/10.3390/ijms232214330