Next Article in Journal
Effects of Heme Site (FA1) Ligands Bilirubin, Biliverdin, Hemin, and Methyl Orange on the Albumin Binding of Site I Marker Warfarin: Complex Allosteric Interactions
Previous Article in Journal
The Oncopig as an Emerging Model to Investigate Copper Regulation in Cancer
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 23
Issue 22
10.3390/ijms232214013
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Neutrophil Extracellular Traps Mediate Bovine Endometrial Epithelial Cell Pyroptosis in Dairy Cows with Endometritis

by
Wenxiang Shen
1,2,
Xiaoyu Ma
1,3,
Dan Shao
2,
Xiaohu Wu
1,
Shengyi Wang
1,
Juanshan Zheng
1,2,
Yanan Lv
1,
Xuezhi Ding
1,
Baohua Ma
2,* and
Zuoting Yan
1,*
1
Lanzhou Institute of Husbandry and Pharmaceutical Science, Chinese Academy of Agricultural Science, Lanzhou 730050, China
2
College of Veterinary Medicine, Northwest A&F University, Xianyang 712000, China
3
College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2022, 23(22), 14013; https://doi.org/10.3390/ijms232214013
Submission received: 8 September 2022 / Revised: 3 November 2022 / Accepted: 9 November 2022 / Published: 13 November 2022
(This article belongs to the Special Issue The Research of Neutrophil 2.0)
Browse Figures
Review Reports Versions Notes

Abstract

Neutrophils are involved in the development of endometritis, but it remains unknown how neutrophils induce inflammation and tissue damage. Neutrophil extracellular traps (NETs) clear invading pathogens during infection but induce pyroptosis, leading to inflammation and tissue damage. Thus, our objective was to investigate whether NETs participate in bovine endometrial epithelial cell (BEEC) pyroptosis during endometritis. To confirm this, NETs and caspase-1/4; apoptosis-associated speck-like protein containing a caspase-recruitment domain(ASC); nod-like receptor protein-3 (NLRP3); and gasdermin D N-terminal (GSDMD-N), TNF-a, IL-1β, IL-6, and IL-18 in endometrial tissue were detected. Pathological section and vaginal discharge smears were performed to visually determine endometritis in the uterus. BEECs were stimulated with NETs to induce pyroptosis, which was treated with DNase I against pyroptosis. Caspase-1/4, ASC, NLRP3, GSDMD-N, TNF-a, IL-1β, IL-6, and IL-18 in BEECs were analyzed in endometrial tissue. The results showed that NET formation, as well as pyroptosis-related proteins and proinflammatory, cytokines were elevated in the endometrial tissue of cows with endometritis. Pathological sections and vaginal discharge smears showed increased neutrophils and plasma cells in the uterus, as well as tissue congestion. In BEECs, NETs increased the level of pyroptosis-related proteins and proinflammatory cytokines and were diminished by DNase I. In summary NETs participate BEEC pyroptosis during endometritis in dairy cows.
Keywords: NETs; pyroptosis; dairy cow; endometritis; BEECs NETs; pyroptosis; dairy cow; endometritis; BEECs

Share and Cite

MDPI and ACS Style

Shen, W.; Ma, X.; Shao, D.; Wu, X.; Wang, S.; Zheng, J.; Lv, Y.; Ding, X.; Ma, B.; Yan, Z. Neutrophil Extracellular Traps Mediate Bovine Endometrial Epithelial Cell Pyroptosis in Dairy Cows with Endometritis. Int. J. Mol. Sci. 2022, 23, 14013. https://doi.org/10.3390/ijms232214013

AMA Style

Shen W, Ma X, Shao D, Wu X, Wang S, Zheng J, Lv Y, Ding X, Ma B, Yan Z. Neutrophil Extracellular Traps Mediate Bovine Endometrial Epithelial Cell Pyroptosis in Dairy Cows with Endometritis. International Journal of Molecular Sciences. 2022; 23(22):14013. https://doi.org/10.3390/ijms232214013

Chicago/Turabian Style

Shen, Wenxiang, Xiaoyu Ma, Dan Shao, Xiaohu Wu, Shengyi Wang, Juanshan Zheng, Yanan Lv, Xuezhi Ding, Baohua Ma, and Zuoting Yan. 2022. "Neutrophil Extracellular Traps Mediate Bovine Endometrial Epithelial Cell Pyroptosis in Dairy Cows with Endometritis" International Journal of Molecular Sciences 23, no. 22: 14013. https://doi.org/10.3390/ijms232214013

APA Style

Shen, W., Ma, X., Shao, D., Wu, X., Wang, S., Zheng, J., Lv, Y., Ding, X., Ma, B., & Yan, Z. (2022). Neutrophil Extracellular Traps Mediate Bovine Endometrial Epithelial Cell Pyroptosis in Dairy Cows with Endometritis. International Journal of Molecular Sciences, 23(22), 14013. https://doi.org/10.3390/ijms232214013

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop