MnASI1 Mediates Resistance to Botrytis cinerea in Mulberry (Morus notabilis)
Abstract
:1. Introduction
2. Results
2.1. Bioinformatics Analyses of MnASIs
2.2. B. cinerea-Induced MnASIs Expression
2.3. Subcellular Localization of MnASI1
2.4. Positive Regulation of MnASI1 for Resistance to B. cinerea
2.5. Detection of Biochemical Indices
2.6. MnASI1 Transgenic Plants Enhance PR1 Expression
3. Discussions
4. Materials and Methods
4.1. Phylogenetic Tree of ASIs
4.2. Quantitative Real-Time PCR
4.3. Transformation of Arabidopsis
4.4. Transient Expression Analysis of Mulberry Gene Function
4.5. Resistance Analysis of Transgenic Arabidopsis and Mulberry to B. cinerea
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Name | Gene Name | GenBank Acc. | CDS (bp) | Size (aa) | MW (kDa) | Predicted pI |
---|---|---|---|---|---|---|
MnASI1 | L484_010983 | EXB74706.1 | 576 | 191 | 20.82 | 4.46 |
MnASI2 | L484_010984 | EXB74707.1 | 603 | 200 | 21.7 | 5.41 |
MnASI3 | L484_010986 | EXB74709.1 | 624 | 207 | 22.65 | 8.35 |
MnASI4 | L484_010988 | EXB74711.1 | 579 | 192 | 21.19 | 7.52 |
MnASI5 | L484_010987 | EXB74710.1 | 576 | 191 | 21.12 | 4.93 |
MnASI6 | L484_010989 | EXB74712.1 | 579 | 192 | 21.24 | 8.53 |
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Wang, D.; Gong, N.; Liu, C.; Li, S.; Guo, Z.; Wang, G.; Shang, Q.; Wang, D.; Ji, X.; Xin, Y. MnASI1 Mediates Resistance to Botrytis cinerea in Mulberry (Morus notabilis). Int. J. Mol. Sci. 2022, 23, 13372. https://doi.org/10.3390/ijms232113372
Wang D, Gong N, Liu C, Li S, Guo Z, Wang G, Shang Q, Wang D, Ji X, Xin Y. MnASI1 Mediates Resistance to Botrytis cinerea in Mulberry (Morus notabilis). International Journal of Molecular Sciences. 2022; 23(21):13372. https://doi.org/10.3390/ijms232113372
Chicago/Turabian StyleWang, Donghao, Na Gong, Chaorui Liu, Suxia Li, Zhaocheng Guo, Gefan Wang, Qiqi Shang, Dongming Wang, Xianling Ji, and Youchao Xin. 2022. "MnASI1 Mediates Resistance to Botrytis cinerea in Mulberry (Morus notabilis)" International Journal of Molecular Sciences 23, no. 21: 13372. https://doi.org/10.3390/ijms232113372