Next Article in Journal
Sugar Metabolism and Transcriptome Analysis Reveal Key Sugar Transporters during Camellia oleifera Fruit Development
Next Article in Special Issue
Brassinosteroids (BRs) Role in Plant Development and Coping with Different Stresses
Previous Article in Journal
Exosomal and Plasma Non-Coding RNA Signature Associated with Urinary Albumin Excretion in Hypertension
Previous Article in Special Issue
Brassinosteroids and the Tolerance of Cereals to Low and High Temperature Stress: Photosynthesis and the Physicochemical Properties of Cell Membranes
Article

Brassinosteroids Mitigate Cadmium Effects in Arabidopsis Root System without Any Cooperation with Nitric Oxide

1
Department of Environmental Biology, Sapienza University of Rome, 00185 Rome, Italy
2
Department of Biosciences, University of Milan, 20133 Milan, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Jana Oklestkova
Int. J. Mol. Sci. 2022, 23(2), 825; https://doi.org/10.3390/ijms23020825
Received: 23 December 2021 / Revised: 9 January 2022 / Accepted: 10 January 2022 / Published: 13 January 2022
(This article belongs to the Special Issue Plant Hormones Brassinosteroids and Its Actions in Plants)
The heavy metal cadmium (Cd) affects root system development and quiescent center (QC)-definition in Arabidopsis root-apices. The brassinosteroids-(BRs)-mediated tolerance to heavy metals has been reported to occur by a modulation of nitric oxide (NO) and root auxin-localization. However, how BRs counteract Cd-action in different root types is unknown. This research aimed to find correlations between BRs and NO in response to Cd in Arabidopsis’s root system, monitoring their effects on QC-definition and auxin localization in root-apices. To this aim, root system developmental changes induced by low levels of 24-epibrassinolide (eBL) or by the BR-biosynthesis inhibitor brassinazole (Brz), combined or not with CdSO4, and/or with the NO-donor nitroprusside (SNP), were investigated using morpho-anatomical and NO-epifluorescence analyses, and monitoring auxin-localization by the DR5::GUS system. Results show that eBL, alone or combined with Cd, enhances lateral (LR) and adventitious (AR) root formation and counteracts QC-disruption and auxin-delocalization caused by Cd in primary root/LR/AR apices. Exogenous NO enhances LR and AR formation in Cd-presence, without synergism with eBL. The NO-signal is positively affected by eBL, but not in Cd-presence, and BR-biosynthesis inhibition does not change the low NO-signal caused by Cd. Collectively, results show that BRs ameliorate Cd-effects on all root types acting independently from NO. View Full-Text
Keywords: adventitious roots; auxin localization; brassinazole; CdSO4; epibrassinolide; lateral roots; nitric oxide epifluorescence; nitroprusside; quiescent center; root apices adventitious roots; auxin localization; brassinazole; CdSO4; epibrassinolide; lateral roots; nitric oxide epifluorescence; nitroprusside; quiescent center; root apices
Show Figures

Figure 1

MDPI and ACS Style

Della Rovere, F.; Piacentini, D.; Fattorini, L.; Girardi, N.; Bellanima, D.; Falasca, G.; Altamura, M.M.; Betti, C. Brassinosteroids Mitigate Cadmium Effects in Arabidopsis Root System without Any Cooperation with Nitric Oxide. Int. J. Mol. Sci. 2022, 23, 825. https://doi.org/10.3390/ijms23020825

AMA Style

Della Rovere F, Piacentini D, Fattorini L, Girardi N, Bellanima D, Falasca G, Altamura MM, Betti C. Brassinosteroids Mitigate Cadmium Effects in Arabidopsis Root System without Any Cooperation with Nitric Oxide. International Journal of Molecular Sciences. 2022; 23(2):825. https://doi.org/10.3390/ijms23020825

Chicago/Turabian Style

Della Rovere, Federica, Diego Piacentini, Laura Fattorini, Nicoletta Girardi, Dario Bellanima, Giuseppina Falasca, Maria M. Altamura, and Camilla Betti. 2022. "Brassinosteroids Mitigate Cadmium Effects in Arabidopsis Root System without Any Cooperation with Nitric Oxide" International Journal of Molecular Sciences 23, no. 2: 825. https://doi.org/10.3390/ijms23020825

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop