BMP3 Affects Cortical and Trabecular Long Bone Development in Mice
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Department of Anatomy and Clinical Anatomy, School of Medicine, University of Zagreb, 10000 Zagreb, Croatia
2
Laboratory for Mineralized Tissues, Center for Translational and Clinical Research, School of Medicine, University of Zagreb, 10000 Zagreb, Croatia
*
Author to whom correspondence should be addressed.
Academic Editor: Kazuo Yudoh
Int. J. Mol. Sci. 2022, 23(2), 785; https://doi.org/10.3390/ijms23020785
Received: 8 December 2021
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Revised: 5 January 2022
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Accepted: 9 January 2022
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Published: 12 January 2022
(This article belongs to the Special Issue Bone Development and Regeneration 2.0)
Bone morphogenetic proteins (BMPs) have a major role in tissue development. BMP3 is synthesized in osteocytes and mature osteoblasts and has an antagonistic effect on other BMPs in bone tissue. The main aim of this study was to fully characterize cortical bone and trabecular bone of long bones in both male and female Bmp3−/− mice. To investigate the effect of Bmp3 from birth to maturity, we compared Bmp3−/− mice with wild-type littermates at the following stages of postnatal development: 1 day (P0), 2 weeks (P14), 8 weeks and 16 weeks of age. Bmp3 deletion was confirmed using X-gal staining in P0 animals. Cartilage and bone tissue were examined in P14 animals using Alcian Blue/Alizarin Red staining. Detailed long bone analysis was performed in 8-week-old and 16-week-old animals using micro-CT. The Bmp3 reporter signal was localized in bone tissue, hair follicles, and lungs. Bone mineralization at 2 weeks of age was increased in long bones of Bmp3−/− mice. Bmp3 deletion was shown to affect the skeleton until adulthood, where increased cortical and trabecular bone parameters were found in young and adult mice of both sexes, while delayed mineralization of the epiphyseal growth plate was found in adult Bmp3−/− mice.
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Keywords:
Bmp3; micro-CT; long bone; cortical bone; growth plate
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MDPI and ACS Style
Banovac, I.; Grgurevic, L.; Rumenovic, V.; Vukicevic, S.; Erjavec, I. BMP3 Affects Cortical and Trabecular Long Bone Development in Mice. Int. J. Mol. Sci. 2022, 23, 785. https://doi.org/10.3390/ijms23020785
AMA Style
Banovac I, Grgurevic L, Rumenovic V, Vukicevic S, Erjavec I. BMP3 Affects Cortical and Trabecular Long Bone Development in Mice. International Journal of Molecular Sciences. 2022; 23(2):785. https://doi.org/10.3390/ijms23020785
Chicago/Turabian StyleBanovac, Ivan, Lovorka Grgurevic, Viktorija Rumenovic, Slobodan Vukicevic, and Igor Erjavec. 2022. "BMP3 Affects Cortical and Trabecular Long Bone Development in Mice" International Journal of Molecular Sciences 23, no. 2: 785. https://doi.org/10.3390/ijms23020785
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