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Article

Disruption of OsPHD1, Encoding a UDP-Glucose Epimerase, Causes JA Accumulation and Enhanced Bacterial Blight Resistance in Rice

1
State Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 311401, China
2
Northern Center of China National Rice Research Institute, China National Rice Research Institute, Shuangyashan 155100, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally for this work.
Academic Editor: Zsófia Bánfalvi
Int. J. Mol. Sci. 2022, 23(2), 751; https://doi.org/10.3390/ijms23020751
Received: 9 December 2021 / Revised: 5 January 2022 / Accepted: 6 January 2022 / Published: 11 January 2022
(This article belongs to the Special Issue Molecular Genetics of Rice Disease Resistance)
Lesion mimic mutants (LMMs) have been widely used in experiments in recent years for studying plant physiological mechanisms underlying programmed cell death (PCD) and defense responses. Here, we identified a lesion mimic mutant, lm212-1, which cloned the causal gene by a map-based cloning strategy, and verified this by complementation. The causal gene, OsPHD1, encodes a UDP-glucose epimerase (UGE), and the OsPHD1 was located in the chloroplast. OsPHD1 was constitutively expressed in all organs, with higher expression in leaves and other green tissues. lm212-1 exhibited decreased chlorophyll content, and the chloroplast structure was destroyed. Histochemistry results indicated that H2O2 is highly accumulated and cell death is occurred around the lesions in lm212-1. Compared to the wild type, expression levels of defense-related genes were up-regulated, and resistance to bacterial pathogens Xanthomonas oryzae pv. oryzae (Xoo) was enhanced, indicating that the defense response was activated in lm212-1, ROS production was induced by flg22, and chitin treatment also showed the same result. Jasmonic acid (JA) and methyl jasmonate (MeJA) increased, and the JA signaling pathways appeared to be disordered in lm212-1. Additionally, the overexpression lines showed the same phenotype as the wild type. Overall, our findings demonstrate that OsPHD1 is involved in the regulation of PCD and defense response in rice. View Full-Text
Keywords: rice (Oryza sativa L.); lesion mimic mutant; programmed cell death; defense response rice (Oryza sativa L.); lesion mimic mutant; programmed cell death; defense response
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MDPI and ACS Style

Gao, Y.; Xiang, X.; Zhang, Y.; Cao, Y.; Wang, B.; Zhang, Y.; Wang, C.; Jiang, M.; Duan, W.; Chen, D.; Zhan, X.; Cheng, S.; Liu, Q.; Cao, L. Disruption of OsPHD1, Encoding a UDP-Glucose Epimerase, Causes JA Accumulation and Enhanced Bacterial Blight Resistance in Rice. Int. J. Mol. Sci. 2022, 23, 751. https://doi.org/10.3390/ijms23020751

AMA Style

Gao Y, Xiang X, Zhang Y, Cao Y, Wang B, Zhang Y, Wang C, Jiang M, Duan W, Chen D, Zhan X, Cheng S, Liu Q, Cao L. Disruption of OsPHD1, Encoding a UDP-Glucose Epimerase, Causes JA Accumulation and Enhanced Bacterial Blight Resistance in Rice. International Journal of Molecular Sciences. 2022; 23(2):751. https://doi.org/10.3390/ijms23020751

Chicago/Turabian Style

Gao, Yu, Xiaojiao Xiang, Yingxin Zhang, Yongrun Cao, Beifang Wang, Yue Zhang, Chen Wang, Min Jiang, Wenjing Duan, Daibo Chen, Xiaodeng Zhan, Shihua Cheng, Qunen Liu, and Liyong Cao. 2022. "Disruption of OsPHD1, Encoding a UDP-Glucose Epimerase, Causes JA Accumulation and Enhanced Bacterial Blight Resistance in Rice" International Journal of Molecular Sciences 23, no. 2: 751. https://doi.org/10.3390/ijms23020751

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