Mutation and Interaction Analysis of the Glycoprotein D and L and Thymidine Kinase of Pseudorabies Virus
Abstract
:1. Introduction
2. Results
2.1. The Amino Acid Sequences of PRV Strains in China Are Different from That Reported Abroad
2.2. Mutations in PRV gD and gL Proteins May Affect the Recognition of Antigenic Epitopes
2.3. The Mutations of gD, gL, and TK Proteins Did Not Affect Their Spatial Conformation but May Affect Their Function
2.4. PRV JL-CC gD Protein Can Bind to Human Nectin-1, Nectin-2, and HS
3. Discussion
4. Materials and Methods
4.1. Virus Strain Information and Multiple Alignments
4.2. Phylogenetic Tree
4.3. Functional Domain and Epitope Analysis of gD, gL, and TK Proteins
4.4. Three-Dimensional (3D) Structures Modeling
4.5. The Mutation Analysis of the gD, gL, and TK Proteins
4.6. Molecular Docking
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Protein | Domain/Family | T Cell Epitopes | B Cell Epitopes | ||||||
---|---|---|---|---|---|---|---|---|---|
Bartha | hSD-1/2019 | JL-CC | Bartha | hSD-1/2019 | JL-CC | Bartha | hSD-1/2019 | JL-CC | |
gD | 56–181 | A69V S82N | A69V S82N | Y35TESWQLTL43, G358TAMGALLV356, H230REVVNYWY238 | Q76VDRLLSEAV86, R165RLVSVDGVN174, I122FGRCRRRTT131, A70LISDPQVDR79, S169VDGVNILTD178 | S82N | S82N | ||
gL | 70–138 | T84N I122V | T84N I122V | V86PSVVVKPY94, M83TAVTSVVV91 | H58PLLGLEPPV67 R44APRREELEW53 | A45H G62D | A45H G62D | ||
TK | 10–278 | T215V | T215V |
Clash | Buried Hydrophilic Introduced | Buried Charge Introduced and Switch | Secondary Structure Altered | Disallowed phi/psi | Buried Charge Replaced | Buried H-Bond Breakage | Cavity Altered | Buried/Exposed Switch | |
---|---|---|---|---|---|---|---|---|---|
gD A59V | +++ | +++ | +++ | NA | −−− | −−− | −−− | ++− | +−− |
gD S82N | +−− | ++− | ++− | NA | −−− | ++− | ++− | −−− | ++− |
gL T84N | +−− | +−− | +−− | NA | −−− | −−− | −−− | −−− | ++− |
gL I122V | +−− | −−− | −−− | NA | +−− | −−− | −−− | −−− | −−− |
TK T215V | −−− | +++ | ++− | NA | −−− | ++− | −−− | −−− | ++− |
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Li, X.; Chen, S.; Zhang, L.; Zheng, J.; Niu, G.; Yang, L.; Zhang, X.; Ren, L. Mutation and Interaction Analysis of the Glycoprotein D and L and Thymidine Kinase of Pseudorabies Virus. Int. J. Mol. Sci. 2022, 23, 11597. https://doi.org/10.3390/ijms231911597
Li X, Chen S, Zhang L, Zheng J, Niu G, Yang L, Zhang X, Ren L. Mutation and Interaction Analysis of the Glycoprotein D and L and Thymidine Kinase of Pseudorabies Virus. International Journal of Molecular Sciences. 2022; 23(19):11597. https://doi.org/10.3390/ijms231911597
Chicago/Turabian StyleLi, Xue, Si Chen, Liying Zhang, Jiawei Zheng, Guyu Niu, Lin Yang, Xinwei Zhang, and Linzhu Ren. 2022. "Mutation and Interaction Analysis of the Glycoprotein D and L and Thymidine Kinase of Pseudorabies Virus" International Journal of Molecular Sciences 23, no. 19: 11597. https://doi.org/10.3390/ijms231911597