Improved Salinity Tolerance-Associated Variables Observed in EMS Mutagenized Wheat Lines
Abstract
:1. Introduction
2. Results
2.1. Selection of Mutagenized Lines for In-Depth Study
2.2. Mutagenized Wheat Lines Differed in Shoot Size in Response to Salinity
Smoothed Estimated Plant Biomass (sEPB) Absolute Growth Rate (AGR)
2.3. Large Variation Observed in Leaf Na+, K+, and Cl− Content on a Dry Weight Basis within the Selected Mutagenized Lines
2.3.1. K+ Content Based on Dry Weight
2.3.2. Na+ Content Based on Dry Weight
2.3.3. Cl− Content Based on the Dry Weight
2.3.4. Leaf Na+:K+ Ratio
2.4. The Water Use Index (sWUI) Was Greater in Two Lines, whereas One Line Was Less Efficient
2.5. Several Lines Had Higher TKW Than the Control at Higher Salt Levels
3. Discussion
3.1. OA6 and OA62 Had Improved Growth during Osmotic and Ion Stress
3.2. Increased Biomass of OA6 and OA62 Correlated Well with Efficient Water Use
3.3. Ion Concentration/Homeostasis
3.3.1. OA23 Retained High Na+ Levels and Maintained Performance
3.3.2. Cl− Levels Were Low in All Mutant Lines Relative to the Control
3.3.3. K+ Levels Were Generally Higher Than the Control except for OA23
3.3.4. Ion Ratios—Increased K+ Levels Manage Na+ Accumulation in Three Lines
3.4. Mutagenized Lines Overall Had Relatively High TKW Relative to the Controls in Saline Conditions
3.5. Variation in the Improved Salinity Tolerance-Associated Variables Observed in the Characterized Lines
4. Materials and Methods
4.1. Plant Material
4.2. Experiment Description
4.3. Preparation of Image Data
4.4. Water Use
4.5. Harvest (Leaf) Data
4.6. Measurement of Leaf Ion Concentration
4.7. Thousand Kernel Weight (TKW)
4.8. Statistical Analyses
4.9. Rationale behind Selection of Lines for in Depth Studies
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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NaCl /Line | BARI | OA6 | OA10 | OA23 | OA25 | OA52 | OA62 |
---|---|---|---|---|---|---|---|
0 mM | 51.65 ± 0.85 | 53.82 ± 4.40 | 29.50 ± 1.29 | 51.84 ± 3.45 | 54.54 ± 1.89 | 52.79 ± 0.90 | 46.48 ± 3.62 |
40 mM | 47.25 ± 2.41 | 45.82 ± 8.66 | 26.27 ± 2.43 | 57.98 ± 7.94 | 51.90 ± 5.06 | 46.56 ± 4.51 | 44.75 ± 0.90 |
80 mM | 48.60 ± 5.28 | 49.73 ± 12.6 | 28.76 ± 2.40 | 62.43 ± 5.58 | 50.21 ± 5.55 | 50.43 ± 5.91 | 44.05 ± 2.42 |
120 mM | 40.81 ± 3.41 | 47.74 ± 2.93 | 26.15 ± 1.51 | 51.13 ± 4.68 | 58.11 ± 0.19 | 58.26 ± 21.3 | 41.57 ± 2.10 |
160 mM | 42.36 ± 4.11 | 46.76 ± 2.82 | 25.86 ± 2.46 | 55.42 ± 8.03 | 47.20 ± 5.06 | 42.87 ± 3.97 | 40.89 ± 1.24 |
Mean | 46.13 | 48.77 | 27.31 | 55.76 | 52.39 | 50.18 | 43.55 |
Group | B | AB | C | A | A | AB | B |
Parameter/Line | OA6 | OA10 | OA23 | OA25 | OA52 | OA62 |
---|---|---|---|---|---|---|
sEPB AGR | ||||||
K+ | ||||||
Na+ | ||||||
Cl− | ||||||
Na+:K+ | ||||||
sWUI | ||||||
TKW |
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Lethin, J.; Byrt, C.; Berger, B.; Brien, C.; Jewell, N.; Roy, S.; Mousavi, H.; Sukumaran, S.; Olsson, O.; Aronsson, H. Improved Salinity Tolerance-Associated Variables Observed in EMS Mutagenized Wheat Lines. Int. J. Mol. Sci. 2022, 23, 11386. https://doi.org/10.3390/ijms231911386
Lethin J, Byrt C, Berger B, Brien C, Jewell N, Roy S, Mousavi H, Sukumaran S, Olsson O, Aronsson H. Improved Salinity Tolerance-Associated Variables Observed in EMS Mutagenized Wheat Lines. International Journal of Molecular Sciences. 2022; 23(19):11386. https://doi.org/10.3390/ijms231911386
Chicago/Turabian StyleLethin, Johanna, Caitlin Byrt, Bettina Berger, Chris Brien, Nathaniel Jewell, Stuart Roy, Hesam Mousavi, Selvakumar Sukumaran, Olof Olsson, and Henrik Aronsson. 2022. "Improved Salinity Tolerance-Associated Variables Observed in EMS Mutagenized Wheat Lines" International Journal of Molecular Sciences 23, no. 19: 11386. https://doi.org/10.3390/ijms231911386