Functional Prediction of trans-Prenyltransferases Reveals the Distribution of GFPPSs in Species beyond the Brassicaceae Clade
Abstract
:1. Introduction
2. Results
2.1. Data Collection and Establishment of PTS-Pre for the Prediction of trans-Prenyltransferases
2.2. Validation of PTS-Pre Prediction Using Experimentally Determined trans-PTs Sequences
2.3. Genome Mining of the trans-PT Gene Family Identified More GFPPSs beyond the Brassicaceae Clade
3. Discussion
4. Materials and Methods
4.1. Sequence Similarity Network and Phylogenetic Analysis
4.2. Gene cloning, Expression, and Protein Purification
4.3. In Vitro trans-Prenyltransferase Activity Assays
4.4. Computational Function Annotation Design of PTS-Pre
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Name | Protein ID | Best Template | Sequence Identity (%) | PTS-Pre Predicted | Experimentally Determined |
---|---|---|---|---|---|
GaGGPPSL-1 | XP_017641950 | 1FPS | 45.4 | C15 | C15 |
GaGGPPSL-2 | XP_017620811 | 5E8H | 67.5 | C20 | C20 |
GaGGPPSL-3 | XP_017622622 | 5E8H | 59.9 | C25 | C25 |
GaGGPPSL-4 | XP_017649086 | 3AQ0 | 71.8 | ≥C30 | NA |
GaGGPPSL-5 | XP_017640526 | 3AQ0 | 74.7 | ≥C30 | ≥C30 |
TcGGPPSL-1 | KAB8318528 | 2FOR | 49.8 | C15 | C15 |
TcGGPPSL-2 | KAB8317749 | 3AQ0 | 39.4 | C20 | C10 |
TcGGPPSL-3 | KAB8318557 | 5E8H | 50.9 | C20 | C20 |
TcGGPPSL-4 | KAB8315220 | 5E8H | 51.0 | C20/C25 | C25 |
SaGGPPSL-1 | WP_059416577 | 2FOR | 36.1 | C15 | C15 |
SaGGPPSL-2 | WP_059424690 | 3Q2Q | 43.5 | C20 | NA |
SaGGPPSL-3 | WP_078945674 | 3OYR | 35.2 | C20/C25 | C15 |
Protein ID | Organism | PTS-Pre Predicted | Experimentally Determined |
---|---|---|---|
WP_084743876 | Tolypothrix campylonemoides * | C20/C25 | C25 |
RCJ15756 | Nostoc sp. ATCC 43529 * | C20/C25 | C25 |
WP_100897628 | Nostoc flagelliforme * | C20/C25 | C25 |
XP_009395333 | Musa acuminata | C20/C25 | C20 |
XP_025814700 | Panicum hallii | C25 | C20 |
XP_021311468 | Sorghum bicolor | C25 | C20 |
RCV24163 | Setaria italica | C25 | C20 |
XP_009406992 | Musa acuminata | C25 | C20 |
XP_009386740 | Musa acuminata | C25 | C25 |
RWW69357 | Ensete ventricosum | C25 | C25 |
PIA55534 | Aquilegia coerulea | C25 | C20 |
XP_023881877 | Quercus suber | C25 | NA |
PIA55630 | Aquilegia coerulea | C25 | C25 |
XP_017622622 | Gossypium arboreum | C25 | C25 |
OMO95617 | Corchorus olitorius | C25 | C25 |
XP_021296398 | Herrania umbratica | C25 | C25 |
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Zhang, J.; Ma, Y.; Chen, Q.; Yang, M.; Feng, D.; Zhou, F.; Wang, G.; Wang, C. Functional Prediction of trans-Prenyltransferases Reveals the Distribution of GFPPSs in Species beyond the Brassicaceae Clade. Int. J. Mol. Sci. 2022, 23, 9471. https://doi.org/10.3390/ijms23169471
Zhang J, Ma Y, Chen Q, Yang M, Feng D, Zhou F, Wang G, Wang C. Functional Prediction of trans-Prenyltransferases Reveals the Distribution of GFPPSs in Species beyond the Brassicaceae Clade. International Journal of Molecular Sciences. 2022; 23(16):9471. https://doi.org/10.3390/ijms23169471
Chicago/Turabian StyleZhang, Jing, Yihua Ma, Qingwen Chen, Mingxia Yang, Deyu Feng, Fei Zhou, Guodong Wang, and Chengyuan Wang. 2022. "Functional Prediction of trans-Prenyltransferases Reveals the Distribution of GFPPSs in Species beyond the Brassicaceae Clade" International Journal of Molecular Sciences 23, no. 16: 9471. https://doi.org/10.3390/ijms23169471