Characterization of the SWEET Gene Family in Longan (Dimocarpus longan) and the Role of DlSWEET1 in Cold Tolerance
Abstract
:1. Introduction
2. Results
2.1. Identification and Phylogenetic Analysis of SWEET Proteins in Longan
2.2. Analysis of Transmembrane Domains and Conserved Motifs
2.3. Exon–Intro Organization of DlSWEET Genes
2.4. Cis-Acting Elements in the Promoters of DlSWEETs
2.5. Tissue-Specific Expression Patterns of DlSWEET Genes
2.6. Soluble Sugar and Expression Patterns of DlSWEET Genes during Fruit Development
2.7. Expression Patterns of DlSWEET Genes under Abiotic Stress Condition
2.8. Overexpression of DlSWEET1 Enhances Cold Tolerance in Transgenic Arabidopsis Plants
3. Discussion
4. Materials and Methods
4.1. Plant Materials, Stress Treatments, and Measurement of Soluble Sugars Content
4.2. Identification, Phylogenetic Analysis, and Motifs Prediction of SWEET Protein in Longan
4.3. Cis-Elements Search and Exon–Intron Organization of DlSWEET Genes
4.4. Expression Pattern Analysis of DlSWEET Genes
4.5. RNA Isolation and qRT-PCR Analysis
4.6. Functional Analysis of DlSWEET1-Overexpressing Transgenic Arabidopsis Thaliana
4.7. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene ID | Gene Name | Clade | Location | gDNA (bp) | CDS (bp) | Protein (aa) | PI | MV (KDa) | GRAVY | TMHs |
---|---|---|---|---|---|---|---|---|---|---|
Dlo_004842.1 | DlSWEET1 | I | scaffold132:1222727..1224250(+) | 1524 | 750 | 249 | 9.44 | 27.20 | 0.678 | 7 |
Dlo_011035.1 | DlSWEET2a | I | scaffold208:248691..251666(−) | 2976 | 732 | 243 | 9.46 | 27.10 | 0.77 | 7 |
Dlo_031144.1 | DlSWEET2b | I | scaffold81:587713..595463(−) | 7751 | 1323 | 440 | 9.32 | 49.29 | 0.236 | 6 |
Dlo_001364.1 | DlSWEET3a | I | scaffold105:240059..241538(−) | 1480 | 639 | 212 | 9.24 | 24.07 | 0.509 | 6 |
Dlo_001358.3 | DlSWEET3b | I | scaffold105:129309..133690(−) | 4382 | 726 | 241 | 8.54 | 26.49 | 0.559 | 7 |
Dlo_001362.1 | DlSWEET3c | I | scaffold105:227382..229337(−) | 1956 | 756 | 251 | 9.06 | 28.19 | 0.459 | 7 |
Dlo_016654.1 | DlSWEET4 | II | scaffold32:314489..315798(−) | 1310 | 573 | 190 | 9.54 | 21.48 | 0.729 | 5 |
Dlo_012330.1 | DlSWEET7a | II | scaffold23:111069..112837(−) | 1769 | 714 | 237 | 9.28 | 26.51 | 0.833 | 7 |
Dlo_036665.1 | DlSWEET7b | II | scaffold1269:54809..57866(+) | 3058 | 780 | 260 | 9.1 | 29.08 | 0.767 | 7 |
Dlo_035889.1 | DlSWEET7c | II | scaffold1269:54830..57818(+) | 2989 | 711 | 237 | 9 | 26.66 | 0.914 | 6 |
Dlo_002779.1 | DlSWEET9a | III | scaffold1141:110734..112484(+) | 1751 | 747 | 248 | 9.38 | 27.76 | 0.496 | 7 |
Dlo_002777.1 | DlSWEET9b | III | scaffold1141:86225..87669(+) | 1445 | 636 | 211 | 9.15 | 23.77 | 0.602 | 5 |
Dlo_002778.1 | DlSWEET9c | III | scaffold1141:97643..100738(+) | 3096 | 777 | 258 | 9.28 | 28.98 | 0.416 | 7 |
Dlo_024131.1 | DlSWEET9d | III | scaffold539:83282..84431(+) | 1150 | 765 | 254 | 9.01 | 28.42 | 0.531 | 6 |
Dlo_006037.1 | DlSWEET10a | III | scaffold1436:78359..79825(+) | 1467 | 855 | 284 | 9.4 | 32.10 | 0.603 | 7 |
Dlo_005388.1 | DlSWEET10b | III | scaffold139:126837..128875(−) | 2039 | 630 | 209 | 8.4 | 23.76 | 0.482 | 5 |
Dlo_006035.1 | DlSWEET10c | III | scaffold1436:44684..45953(+) | 1270 | 810 | 269 | 9.17 | 29.76 | 0.645 | 7 |
Dlo_026392.1 | DlSWEET15 | III | scaffold601:142..2474(+) | 2333 | 741 | 246 | 5.77 | 27.80 | 0.574 | 5 |
Dlo_006477.1 | DlSWEET16a | IV | scaffold148:330655..332743(−) | 2089 | 735 | 244 | 8.56 | 27.11 | 0.645 | 6 |
Dlo_006478.1 | DlSWEET16b | IV | scaffold148:336807..338137(−) | 1331 | 423 | 140 | 6.17 | 15.48 | 0.344 | 3 |
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Fang, T.; Rao, Y.; Wang, M.; Li, Y.; Liu, Y.; Xiong, P.; Zeng, L. Characterization of the SWEET Gene Family in Longan (Dimocarpus longan) and the Role of DlSWEET1 in Cold Tolerance. Int. J. Mol. Sci. 2022, 23, 8914. https://doi.org/10.3390/ijms23168914
Fang T, Rao Y, Wang M, Li Y, Liu Y, Xiong P, Zeng L. Characterization of the SWEET Gene Family in Longan (Dimocarpus longan) and the Role of DlSWEET1 in Cold Tolerance. International Journal of Molecular Sciences. 2022; 23(16):8914. https://doi.org/10.3390/ijms23168914
Chicago/Turabian StyleFang, Ting, Ya Rao, Mengzhen Wang, Yun Li, Yujun Liu, Pengpeng Xiong, and Lihui Zeng. 2022. "Characterization of the SWEET Gene Family in Longan (Dimocarpus longan) and the Role of DlSWEET1 in Cold Tolerance" International Journal of Molecular Sciences 23, no. 16: 8914. https://doi.org/10.3390/ijms23168914